Figure 3
From: Dictyostelium cells bind a secreted autocrine factor that represses cell proliferation
Wild-type conditioned growth medium slows cell proliferation. Conditioned growth medium was collected and diluted with fresh growth medium to the indicated concentrations. Wild-type (WT) and aprA- cells were then grown in the mixed media, and cells were counted after 12 hours. Values are mean ± SEM (n = 3). The absence of error bars indicates that the error was smaller than the plot symbol. Lines are curve fits of a sigmoidal dose response curve; the dashed line is the fit to aprA-. The inhibition of WT and aprA- proliferation is significant with p < 0.05 at 30% and higher conditioned growth medium concentrations (1-way ANOVA, Dunnett's test).