The acidic domains of the Toc159 chloroplast preprotein receptor family are intrinsically disordered protein domains

Table 1 Secondary structure composition of 159A_His and 132A_His under different experimental conditions^a

Protein and experimental condition	α-helix	β-sheet	Random coil
132A 25°C, pH 8	0.03	0.22	0.76
132A 5°C (pH 8)	0.04	0.33	0.63
132A 60°C (pH 8)	0.07	0.43	0.49
132A pH 3 (25°C)	0.25	0.20	0.55
132A pH 10 (25°C)	0.03	0.21	0.76
132A 10% TFE	0.04	0.30	0.66
132A 50% TFE	0.28	0.28	0.44
159A 25°C, pH 8	0.05	0.32	0.63
159A 5°C (pH 8)	0.04	0.31	0.65
159A 60°C (pH 8)	0.07	0.38	0.55
159A pH 3 (25°C)	0.08	0.41	0.51
159A pH 10 (25°C)	0.04	0.22	0.75
159A 10% TFE	0.08	0.40	0.52
159A 50% TFE	0.28	0.20	0.52

^aDeconvolution of the far-UV CD spectra for 159A_His and 132A_His was performed using the K2D method. Values represent the proportion of secondary structure composition.

ISSN: 1471-2091