Table 1 Identification of endonuclein interacting proteins by peptide sequencing using tandem mass spectrometry.
Protein spot No. | Peptide sequence obtained by tandem mass spectrometry | Identification of protein | Localization | Mr (kDa) | pI |
|---|---|---|---|---|---|
1 | ELEEIVQPIISK | BiP/Grp78 | ER resident | 72.1 | 4.9 |
2 | VSEGGPAEIAGLQIGDK | TIP-1 | Cytoplasm* Nucleus | 12.9 | 9.2 |
4 | TLEVEIEPGVR | DNAJB11/HEDJ (EIP-4) | ER/secreted | 40.5 | 5.8 |
8 | TGAELVTCGSVLK | SDF2-like protein 1 (EIP-8) | ER resident | 23.6 | 6.6 |
10 | Failed | Â | Â | Â | Â |
11 | VSEGGPAEIAGLQIGDK | TIP-1 | Cytoplasm* Nucleus | 12.9 | 9.2 |
16 | SQIFSTASDNQPTVTIK TFDLTGI LTPEEIER NELESYAYSLK KKELEEIVQPIISK | BiP/Grp78 | ER resident | 72.1 | 4.9 |
17 | SGDSEVYQLGDVSQK VGQDPVLR | Mt-SSB (ssDNA binding protein/P16) | Mitochondria Nucleus* Cytoplasm | 17.2 | 10.5 |
18 | ENKDVLTFTCEPK ILILPSVTR LSIPQITTK LFIPQITTK | Pregnancy specific ÎČ-1 glycoproteins (PSBGs) | ER/secreted | 36.2-52.7 | 8.0-9.3 |