Figure 1

Affinity purification of endonuclein interacting proteins. A placental tissue extract was applied to a column with immobilized endonuclein, (A), and to a parallel column without endonuclein, (B), in a physiologic buffer. Proteins were eluted from both columns at pH 2.7. The eluates were neutralized and finally subjected to two-dimensional gel electrophoresis with subsequent silver staining. Arrows with numbers indicate protein spots that were excised from the gel and subjected to nano-electrospray tandem mass spectrometry for identification. The partial sequences and the names of the identified proteins are shown in Table 1. Proteins that interacted unspecifically with the column matrix are given with arrowheads.