Biochemical evidence for the tyrosine involvement in cationic intermediate stabilization in mouse β-carotene 15, 15'-monooxygenase

Table 1 Biochemical Characteristics of wt BCMO1 and enzymatically active mutants^a.

^aK_m [μM] and V_max [pmol retinal/hour* μg enzyme] were calculated from Hanes [s/v vs s] plots. Calculation of enzymatic activity was based on the production of retinal from β-carotene. Data was calculated based on average substrate curve for each protein.
^# These data are K_m apparent values due to limitations of β-carotene solubility in reaction mixture (the highest concentration of β-carotene is 42 μM).

ISSN: 1471-2091