Table 5 Effect of various inhibitors on CPRHG activity
From: Purification and characterization of cysteine protease from germinating cotyledons of horse gram
Inhibitor | Conc. | Relative activity (%) |
|---|---|---|
pCMB | 10 mM | 0 |
NEM | 100 mM | 61 |
Iodoacetamide | 100 mM | 8 |
DTNB | 0.2% | 40 |
DIPF | 10 mM | 93 |
STI | 0.05% | 99 |
Eserine | 0.01% | 96 |
pMSF | 100 mM | 81 |
Bestatin | 0.1 mg/ml | 92 |
Aprotinin | 0.001% | 96 |
Pepstatin | 0.01% | 88 |
DEPC | 10 mM | 0 |
EDTA | 10 mM | 95 |
1, 10-Phenanthroline | 10 mM | 99 |
Control | - | 100 |