Skip to main content
Figure 1 | BMC Biochemistry

Figure 1

From: Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats

Figure 1

Co-immunoprecipitation of endogenous PP6R3 and PP6c. (A) Immunoprecipitates (IP) were prepared from HeLa cells with pre-immune sheep serum (lane 1), blank beads (lane 2) or anti-PP6R3 sheep antibody (lane 3). Precipitates were analyzed by immunoblotting for PP6R3 (upper panel) and PP6c (lower panel). (B) Co-immunoprecipitation of PP6R3 and PP6c from HepG2 cells with anti-PP6R3 antibody (lane 1). The PP6R3 and PP6c remaining unbound were detected by immunoblotting the supernatant (S, lane 2) for PP6R3 (upper panel) and PP6c (lower panel)

Back to article page