Table 1 Primers used for cloning psrpk and tps
DNA fragmants | sense and anti-sense primers | |
|---|---|---|
psrpk | 1~1278 | F1:5'-CCGGAATTCT ATGGAAAACATATTCAAGGAGAAGG-3' R1: 5'-ACGCGTCGAC AGAAATGGAGGCACATCAGCC-3' |
tp1 | 84~1278 | 5'-CCGGAATTCT ATGGATAGCGAAGATGAGGGAAC-3'/R1 |
tp2 | 1~588 | F1/5'-ACGCGTCGAC AGCACGTTTTCAGGTTTGAG-3' |
tp3 | 589~1278 | 5'-CCGGAATTCT ATGGACCATCTGTTACGACCAG-3'/R1 |
tp4 | 778~1278 | 5'-CCGGAATTCT ATGAAAATCGCCGATCTAGGCAC-3'/R1 |
tp5 | 589~951 | F2: 5'-CCGGAATTCATGGACCATCTGTTACGACCAGACAC-3' R2: 5'-ACGCGTCGACTGGCAAAAAAGGAGATCTCCGGTGG-3' |
tp6 | 589~1029 | F2/5'-ACGCGTCGACTGGCGCGGCATTCTTCCTAGTAGCTC-3' |
tp7 | 985~1278 | F3: 5'-ACGCGTCGACGATGGATCACTTGGCTTTGATGATTG-3' R3: 5'-ATGCGGATCC GCCAGAAATGGAGGCACATCAGCC-3' |
tp8 | 589~951 985~1278 | F2/R2 F3/R3 |
tp9 | 1~951 985~1278 | F4: 5'-CCGGAATTC ATGGAAAACATATTCAAGGAGAAG-3'/R2 F3/R3 |