Figure 3
From: Human Rad51 mediated DNA unwinding is facilitated by conditions that favour Rad51-dsDNA aggregation
RAD52 stimulates RAD51 mediated dsDNA unwinding even in presence of ADP: Time-course analysis. Relaxed ĻX174 DNA (30 Ī¼M) along with Topo I was incubated with RAD51 (3.0 Ī¼M) in presence of RAD52 (2.0 Ī¼M) in binding buffer R at 37Ā°C with either 1 mM ATP (Panel A) or ADP (Panel B). Reactions were terminated after indicated time points by SDS/Proteinase K treatment and analyzed on agarose gels. Lane 6 in both Panels represents only RAD52 control while lane 7 represents only RAD51 control. Percentage intensity of unwound DNA to total DNA per lane (as explained earlier) was plotted as a function of time (Panel C).