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Table 1 List of mapped DNA cleavage sites

From: Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol

Cleaved sequencesa

Substrate

 

-6

-5

-4

-3

-2

-1

1

2

3

4

5

6

 

Sequences cleaved by both EcTOP and MtTOP

             

Sequences preferred equally

             
 

G

G

C

A

T

T

T

C

G

G

T

G

MdnaK

 

C

T

C

G

C

A

A

G

C

C

G

T

MdnaK

 

T

T

C

C

A

A

C

G

G

A

T

C

MdnaK

 

T

T

C

C

T

T

G

A

C

C

A

C

MdnaK

 

T

G

C

C

A

T

A

T

T

T

C

G

PBAD

 

T

T

C

A

A

G

C

T

T

G

T

C

PBAD

 

C

T

C

G

T

C

T

T

C

C

A

T

Mtop

 

T

T

C

C

A

T

C

G

C

C

G

C

Mtop

 

G

A

C

C

G

A

A

A

T

G

C

T

Mtop

MtTOP preferred sequences b

             
 

T

T

C

C

A

G

T

C

G

G

T

G

MdnaK

 

G

A

C

C

G

C

A

C

T

C

G

C

MdnaK

 

C

A

C

G

T

T

G

T

G

C

T

C

MdnaK

 

T

T

C

A

C

C

T

C

G

C

C

C

MdnaK

 

A

T

C

G

T

C

A

T

C

A

C

C

PBAD

 

T

T

C

A

A

C

T

G

A

C

C

T

PBAD

EcTOP preferred sequences b

             
 

A

G

C

C

C

T

T

C

C

A

G

T

MdnaK

 

C

G

C

A

C

T

C

G

C

A

A

G

MdnaK

 

A

T

C

A

C

G

T

T

G

T

G

C

MdnaK

 

T

T

C

C

T

T

G

C

C

G

C

C

MdnaK

 

G

T

C

A

T

C

A

C

C

G

G

A

PBAD

Sequences cleaved by MtTOP alone

             
 

G

T

C

G

T

C

G

C

C

G

C

C

MdnaK

 

T

T

C

A

G

T

T

T

T

G

C

A

PBAD

 

G

A

C

A

G

T

G

C

A

C

C

C

PBAD

Sequences cleaved by EcTOP alone

             
 

A

A

C

C

T

C

A

T

C

G

G

G

MdnaK

 

C

G

C

C

G

A

G

G

T

G

G

T

MdnaK

 

C

G

C

C

A

C

T

T

C

A

C

C

PBAD

  1. a Sequences are numbered from -6 to 6 (5' to 3'). Topoisomerase I mediated cleavage occurs between -1 and 1.
  2. b Among sequences cleaved by both EcTOP and MtTOP, sequences showing at least one-fold higher cleavage intensity towards EcTOP or MtTOP than the other enzyme are grouped as EcTOP or MtTOP preferred sequences respectively
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BMC Biochemistry

ISSN: 1471-2091

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