Skip to main content
Figure 5 | BMC Biochemistry

Figure 5

From: Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol

Figure 5

Effect of Mg2+ ion concentration on DNA relaxation. Effect of different concentrations of Mg2+ ion concentration ranging from 0–20 mM in DNA relaxation assays containing (A) high level, 1 enzyme unit corresponding to 100 ng of EcTOP and 500 ng of MtTOP; or (B) low level (50 ng) of EcTOP or MtTOP. C: control with no enzyme.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us