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Figure 1 | BMC Biochemistry

Figure 1

From: Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol

Figure 1

Expression of recombinant MtTOP in E. coli Arctic express (DE3)RP strain. SDS PAGE analysis of total cell lysate (lanes 1,3) and soluble cell lysate (lane 2,4) of Arctic express (DE3)RP cells transformed with pLIC-MTOP and induced with 0 mM (lanes 1,2) and 1 mM IPTG (lanes 3,4) at the end of 24 hours of induction in LB at 12°C. M: molecular weight standards.

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BMC Biochemistry

ISSN: 1471-2091

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