Figure 1

Generation of bovine and human TAFIa. Bovine and human TAFI (1 μg) were incubated with increasing amounts of trypsin (A) or thrombin/solulin complex (B) (all values in μg). Proteolysis products were then analyzed by SDS-PAGE and visualized by Coomassie Brilliant Blue staining. Additionally, TAFI (0.2 μg of bovine or human) was incubated with increasing amounts of thrombin/solulin complex (C). Increase in activity of bovine (filled circles) and human (open circles) TAFIa was monitored through HPLC based kinetic assay using Hip-Arg substrate as described in the method section. Note that compared to human TAFI, roughly 15 times less proteinase complex is required to generate 100% active bovine TAFIa.