Figure 4
From: Exploring the functional interaction between POSH and ALIX and the relevance to HIV-1 release
Wild type POSH but not the ubiquitination inactive V14A mutant enhances rescue of virus production by ALIX. A: The left panel shows Virus release, Gag processing and POSH and ALIXexpression analyzed by Western blot. The right panel shows the amounts of infectious units released from the cells analyzed by β-galactosidase quantification after infection of TZM-bl cells. Shown are virus infectivities relative to the HIV-1ΔPTAP control ± SD. HIV-1ΔPTAP-mutant was co-transfected with: lane 1, empty pcDNA3.1V5His; lane 2, a V5-POSH expression plasmid; lane 3, a FLAG-ALIX expression plasmid; lane 4, vectors expressing FLAG-ALIX and V5-POSH; lane 5, a plasmid encoding POSHV14A; lane 6, vectors encoding FLAG-ALIX and POSHV14A. RP0: ribosomal P0 antigen. B: Quantification of Western blot data using AIDA Software (Raytest). The left panel shows the amount of Gag release determined by the ratio of virus associated Gag/total Gag expressed relative to the control set to 1. The right panel shows the processing of Gag in the cell lysates as determined by the ratio of CA/total Gag expressed relative to the control set to 1.