Figure 7
From: Both Ca2+ and Zn2+ are essential for S100A12 protein oligomerization and function
Tyrosine and TNS fluorescence of S100A12/metal complexes. (A) TNS Fluorescence spectra of S100A12-Ca2+, S100A12-Zn2+ and S100A12-Zn2+-Ca2+ complexes in 10 mM pH 7.4 Hepes buffer, at total metal ion concentration of 180 μM (Ca2+) and 200 μM (Zn2+). (B and C) Fluorescence titration of apo-S100A12 first with Ca2+ up to the total concentration of 180 μM and then with Zn2+. S100A12 concentration was 2.5 μM, and TNS concentration was 20 μM.