Figure 6From: Both Ca2+ and Zn2+ are essential for S100A12 protein oligomerization and functionTyrosine and TNS fluorescence of S100A12/metal complexes. (A) Intrinsic tyrosine fluorescence spectra of apo-S100A12 (2.5 μM), and S100A12-Ca2+ and S100A12-Zn2+ complexes in 10 mM pH 7.4 Hepes buffer, at total metal ion concentration of 275 μM and 244 μM, respectively. (B and C) Fluorescence titration of apo-S100A12 with Ca2+ and Zn2+.Back to article page