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Figure 3 | BMC Biochemistry

Figure 3

From: Redox biology of Mycobacterium tuberculosis H37Rv: protein-protein interaction between GlgB and WhiB1 involves exchange of thiol-disulfide

Figure 3

Peptide mass fingerprinting of GlgB. DTT treated and untreated GlgB was alkylated, digested with trypsin and precipitated with TCA. The molecular mass of the peptides were analyzed by MALDI-TOF. (A) Peptide mass spectra highlighting the molecular mass of peptide containing C95 and C658 (marked with arrow) under oxidizing conditions. (B) Peptide mass spectra under reducing conditions. The molecular mass of the peptides containing cysteines (as labeled) were similar to the peptide obtained under oxidized conditions, confirming that both C95 and C658 are free and surface exposed. (C) Peptide mass spectra of GlgB pre-incubated at 45°C before reduction, alkylation and trypsinization. Peptide containing C617 is marked with an arrow; however C193 could not be detected.

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BMC Biochemistry

ISSN: 1471-2091

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