Figure 4

Effect of storage condition on the colour of the activity band developed using NED. After electrophoresis (of 1 U each of AAA on eel AChE and AAA on human serum BChE) under non-denaturing conditions, the gel was stained for AAA activity as described under 'Materials and methods'. The stained gel was then treated with 0.3 M TCA and stored in Tris-HCl Buffer, pH 8.6, where upon the colour of the band changes from purple to brick red (A). The characteristic purple colour, with a blue tint is regained by treating the gel once again with the TCA solution (B).