Figure 2From: Effect of polyamines and synthetic polyamine-analogues on the expression of antizyme (AtoC) and its regulatory genesThe effect of the polyamines PUT and SPD in the transcription of the atoSC two component system genes.(a) The E. coli K12 strain BW25113 (atoSC+) and (b) E. coli K12 strain BW28878(ΔatoSC), were transformed with recombinant plasmids, pCPG5(atoA-lacZ) and pCPG6(atoC-lacZ), carrying the various promoters of the atoSC two component system. Polyamine-mediated induction was measured by assaying β-galactosidase expression in the presence of increasing concentrations of polyamines (0, 0.3, 0.5, 1 and 2.5 mM). The results are presented from three independent experiments, while in each experiment two clones from each transformant were tested.Back to article page