Characterization of a Nudix hydrolase from Deinococcus radiodurans with a marked specificity for (deoxy)ribonucleoside 5'-diphosphates
© Fisher et al; licensee BioMed Central Ltd. 2004
Received: 13 February 2004
Accepted: 17 May 2004
Published: 17 May 2004
Nudix hydrolases form a protein family whose function is to hydrolyse intracellular nucleotides and so regulate their levels and eliminate potentially toxic derivatives. The genome of the radioresistant bacterium Deinococcus radiodurans encodes 25 nudix hydrolases, an unexpectedly large number. These may contribute to radioresistance by removing mutagenic oxidised and otherwise damaged nucleotides. Characterisation of these hydrolases is necessary to understand the reason for their presence. Here, we report the cloning and characterisation of the DR0975 gene product, a nudix hydrolase that appears to be unique to this organism.
The DR0975 gene was cloned and expressed as a 20 kDa histidine-tagged recombinant product in Escherichia coli. Substrate analysis of the purified enzyme showed it to act primarily as a phosphatase with a marked preference for (deoxy)nucleoside 5'-diphosphates (dGDP > ADP > dADP > GDP > dTDP > UDP > dCDP > CDP). Km for dGDP was 110 μM and kcat was 0.18 s-1 under optimal assay conditions (pH 9.4, 7.5 mM Mg2+). 8-Hydroxy-2'-deoxyguanosine 5'-diphosphate (8-OH-dGDP) was also a substrate with a Km of 170 μM and kcat of 0.13 s-1. Thus, DR0975 showed no preference for 8-OH-dGDP over dGDP. Limited pyrophosphatase activity was also observed with NADH and some (di)adenosine polyphosphates but no other substrates. Expression of the DR0975 gene was undetectable in logarithmic phase cells but was induced at least 30-fold in stationary phase. Superoxide, but not peroxide, stress and slow, but not rapid, dehydration both caused a slight induction of the DR0975 gene.
Nucleotide substrates for nudix hydrolases conform to the structure NDP-X, where X can be one of several moieties. Thus, a preference for (d)NDPs themselves is most unusual. The lack of preference for 8-OH-dGDP over dGDP as a substrate combined with the induction in stationary phase, but not by peroxide or superoxide, suggests that the function of DR09075 may be to assist in the recycling of nucleotides under the very different metabolic requirements of stationary phase. Thus, if DR0975 does contribute to radiation resistance, this contribution may be indirect.
The Nudix hydrolases are a family of nucleotide hydrolases found in virtually all organisms. They hydrolyse a wide range of substrates including (d)NTPs, pyridine nucleotides, coenzyme A, dinucleoside polyphosphates and nucleotide sugars, all of which conform to the general structure of a nucleoside diphosphate linked to another moiety X (NDP-X) [1–3]. More recently, non-nucleotide compounds that do not conform to the NDP-X structure, such as diphosphoinositol polyphosphates and phosphoribosyl pyrophosphate, have been added to the range of substrates degraded by some of these enzymes [4–7].
Catalytic activity resides in a distinctive motif, the Nudix (formerly MutT) motif, usually resulting in the cleavage of a pyrophosphate bond. Substrate specificity, which can be relatively broad or narrow, results from interactions with other regions of the protein structure, some of which have been defined. For example, the sequence LLTxR[SA]x3Rx3Gx3FPGG located immediately upstream of the Nudix motif, is found in hydrolases that prefer CoA and CoA esters as substrates [8–10], while the sequence SQPWPFPxS is found a short distance downstream of the Nudix motif in hydrolases characterized as NADH diphosphatases [11–14]. Other motifs may characterise ADP-ribose pyrophosphatases , diadenosine tetraphosphate hydrolases  and UTP pyrophosphohydrolases .
The proposed functions of this family are to eliminate potentially toxic nucleotide metabolites from the cell, e.g. oxidised, mutagenic purine nucleotides in the case of E. coli MutT [16–19] and human NUDT1 (MTH1) [20, 21], and to regulate the concentrations of nucleotide cofactors and signalling molecules for optimal cell growth and survival in response to the cellular environment . The number of genes encoding Nudix hydrolases varies widely, from zero in most Mycoplasma species to around 30 in streptomycetes. This variation correlates fairly closely with genome size and presumably reflects the metabolic capacity and growth or environmental adaptability of the different organisms. However, some bacteria have many more Nudix genes than would be expected from their DNA content. One such is the radiation- and desiccation-resistant Deinococcus radiodurans [22, 23] which has roughly three times as many Nudix genes as would be expected from its genome size of 3.3 Mb [24, 25], suggesting a particular selective pressure on this organism to maintain an expanded set of these genes. In order to understand how the possession of 25 Nudix hydrolases relates to the biology of D. radiodurans, we are studying the gene products, particularly those that have no obvious orthologues in other organisms. Here, we describe a new Nudix hydrolase that has a marked degree of specificity for ribonucleoside and deoxyribonucleoside 5'-diphosphates [(d)NDPs].
Expression and purification of the DR0975 gene product
The DR0975 gene was cloned by PCR from genomic DNA into the pET15b expression vector to yield a 20 kDa N-terminally hexahistidine-tagged protein. This protein was purified by chromatography on a Ni-CAM HC affinity column (Sigma) and the resulting product was judged to be 95% pure (not shown).
Hydrolysis of nucleotide substrates by DR0975 protein. Nucleotides were screened for substrate activity as described in Materials and Methods.
NAD, NADP(H), FAD, CoA
Hydrolysis of (d)NDP substrates by DR0975 protein under optimised conditions. Assays containing 50 mM BisTrisPropane, pH 9.4, 7.5 mM Mg acetate, 3 μg/DR0975 protein and 500 μM substrate were incubated at 37°C for 15 min.
HPLC analysis of the products of dGDP hydrolysis showed them to be dGMP and Pi (results not shown). No further degradation of the dGMP was observed, therefore the enzyme is acting primarily as a nucleoside diphosphate phosphohydrolase (EC126.96.36.199). Since the low activity observed with Ap5A, Ap6A and NADH would require pyrophosphatase activity, degradation of these substrates was checked by colorimetric and HPLC assays and confirmed. In the case of Ap5A, for example, initial products were ADP and ATP, the ADP then being rapidly converted to AMP + Pi (results not shown).
Non-nudix (d)NDP phosphohydrolase activities have previously been described in mammalian tissues, and some of these enzymes are also active towards the structurally similar enzyme cofactor thiamine pyrophosphate [30–32]. Therefore, TPP was tested as a possible substrate for the DR0975 protein, but no activity was detected.
Expression analysis of the DR0975 gene
The DR0975 protein is unusual in its preference for (d)NDPs, showing that X can be H in the commonly used NDP-X substrate designation. Such activity has only recently been observed in one other nudix hydrolase, the human NUDT5 ADP-sugar pyrophosphatase that, surprisingly, is actually more selective for 8-OH-dGDP . However, DR0975 has no activity with any of the NDP-sugars tested and so seems unlikely to be a NUDT5 orthologue. Phosphatase activity among the nudix hydrolases has previously been observed with those active towards diphosphoinositol polyphosphates and phosphoribosyl pyrophosphate [4–7]. Interestingly, these enzymes also act as pyrophosphatases with the alternative diadenosine polyphosphate substrates. Similarly, DR0975 has low activity with some long chain (di)adenosine polyphosphates and with NADH. Determination of the structure of this enzyme should reveal how this is achieved.
The D. radiodurans genome contains 23 nudix genes, two of which encode proteins with two distinct nudix motifs (active sites). It has been suggested that this large number is related to the ability of this organism to withstand high doses of ionizing radiation [24, 25], itself a probable consequence of the desiccation tolerance of the organism . Both ionising radiation and dehydration/rehydration impose severe oxidative stress and might be expected to generate potentially toxic and mutagenic oxidised derivatives within the nucleotide pool. Nudix hydrolases with a degree of specificity for oxidised nucleotide such as 8-OH-dGTP, 2-OH-dATP, 8-OH-dATP, 5-OH-CTP and 8-OH-dGDP have been isolated from E. coli [16, 17, 36, 37] and mammalian cells [20, 28, 38, 39] and so it is possible that some of the additional nudix genes in the D. radiodurans genome encode enzymes active towards other oxidized nucleotides. However, the lack of preference for 8-OH-dGDP compared to dGDP suggests that the former nucleotide is unlikely to be an important substrate for this enzyme in vivo. It is possible, of course, that some other oxidized (d)NDP derivative or derivatives are physiologically relevant substrates and this highlights the problem with the study of new nudix hydrolases – that conclusions are often limited by the availability of suitable, novel substrates. In the case of DR0975, however, the dramatic induction of activity upon entry into stationary phase and the lack of significant induction by peroxide or superoxide suggest an alternative function.
The bacterial stationary phase is characterised by extensive changes in patterns of gene expression leading to physical and morphological adaptations that are designed to maintain viability during starvation [40, 41]. DNA synthesis ceases and there is considerable RNA degradation to supply energy for maintenance metabolism. These changes will inevitably involve nucleotide pools as they are diverted to other activities, such as cell wall synthesis. Thus, the DR0975 protein may have the relatively non-specific function of recycling nucleic acid nucleotides. Alternative, more specific functions can also be imagined. For example, the nucleoside diphosphatase of the mammalian endoplasmic reticulum is believed to eliminate UDP, a product of UDP-glucose:glycoprotein glucosyltransferase . UDP inhibits this enzyme and, if allowed to accumulate, would inhibit protein glucosylation. Thus, although not a nudix hydrolase, this enzyme seems to fulfil the housecleaning role proposed for nudix hydrolases . The D. radiodurans DR0975 protein may serve a similar function in stationary phase bacteria in pathways leading to the synthesis of various NDP-sugars. Hence, DR0975 may be involved in the reprogramming of nucleotide pools to meet the requirements of stationary phase. Stationary phase D. radiodurans are more radiation resistant than logarithmic phase cells , so an indirect contribution of DR0975 to radiation tolerance is conceivable. Such a role may indeed be unique to this organism as a BLAST search reveals no sequences of close similarity to DR0975 among the nudix genes of other sequenced bacterial genomes (>100).
In the absence of evidence to the contrary, we would suggest that a likely role of the DR0975 nucleoside diphosphate phosphohydrolase is to recycle (deoxy)nucleoside diphosphates as part of the general reprogramming of metabolism that occurs during stationary phase. Whether this is true of others among the large number of nudix genes in this organism remains to be determined. Ultimately, an understanding of the roles of the nudix hydrolases of D. radiodurans will require systematic gene disruption and phenotypic analysis. Due to the overlapping substrate specificity of these enzymes, multiple deletions may be required in order to observe a phenotype. This will present an interesting but worthwhile challenge for future research.
8-Hydroxy-2'-deoxyguanosine 5'-diphosphate (8-OH-dGDP) was prepared as described previously . All other nucleotides were from Sigma. Calf intestinal alkaline phosphatase and yeast inorganic pyrophosphatase were from Roche. Nde I, Bam HI and the pET15b expression vector were from Novagen. Pfu DNA polymerase was from Stratagene and M-MLV reverse transcriptase (RNase H minus) was from Promega. Oligonucleotides were from MWG Biotech. TRIzol and DNAse I (Amplification Grade) were from Invitrogen.
Cloning of DR0975 from genomic DNA
The DR0975 coding region was amplified from genomic DNA by PCR using Pfu DNA polymerase, a 33-mer oligonucleotide forward primer d(CGAGGACCCATATGGGGCGGCGTGATCTGCTGG) and 32-mer reverse primer d(GCCCTGCCTGGATCCGCTAGCGGTCCTTGACC). These primers incorporated an Nde I restriction site at the start of the gene, and a Bam HI site at the end. After amplification, the DNA was recovered by phenol-chlorofom extraction and digested with Nde I and Bam HI. The gel-purified restriction fragment was ligated into the appropriate restriction sites of plasmid pET15b and the resulting construct, pET-0975 containing the DR0975 coding region with an upstream His tag sequence under the control of a T7 lac promoter, was used to transform E. coli XL1-Blue cells for propagation.
Protein expression in E. coli and purification
E. coli strain BL21(DE3) was transformed with pET-0975. A single colony was inoculated into 10 ml LB medium containing 60 μg/ml ampicillin and grown overnight at 37°C. The cells were transferred to 1 litre LB medium containing 60 μg/ml ampicillin and grown to an A600 of 0.6. Isopropyl-1-thio-β-D-galactopyranoside was added to 1 mM and the cells induced for 3 h. The cells (4.3 g) were harvested, washed and resuspended in 25 ml breakage buffer (50 mM Tris-HCl, pH 8.0, 300 mM NaCl). The cell suspension was sonicated and centrifuged at 10,000 g for 10 min. The supernatant was recovered and loaded in 10 ml aliquots at 1 ml/min on to a 7 × 50 mm Ni-CAM HC affinity resin column equilibrated with breakage buffer. After elution of unbound protein, a 20 min linear gradient of 0–50 mM histidine in breakage buffer was applied at 1 ml/min. Fractions of 1 ml were collected and analysed by SDS-PAGE. Fractions containing the 20.9 kDa His-tagged DR0975 protein were pooled and DTT was added to a final concentration of 1 mM.
Nucleotide substrates were assayed as previously described  using the following conditions. Assays (200 μl) contained 50 mM Tris-HCl, pH8.0, 5 mM Mg acetate, 1 mM DTT, 100 μM substrate, 0.5 μg inorganic pyrophosphatase or 1 μg/ml alkaline phosphatase as appropriate (except with (d)NDP substrates) and 5 μg DR0975 protein and were incubated at 37°C for 15 mins.
HPLC analysis of dGDP and 8-OH-dGDP hydrolysis
Assay samples (50 μl) were injected directly on to a 2.1 × 100 mm Brownlee AX300 column in buffer A (0.1 M potassium phosphate, pH 6.1) and eluted with a gradient of 0–5% buffer B (4.5 min), 5–40% B (7.3 min), 40% B (13.0 min), where B = 0.1 M potassium phosphate, pH 6.1, 50% (v/v) acetonitrile at a flow rate of 0.5 ml/min.
Expression analysis of the DR0975 gene
Expression of the DR0975 gene was determined in cells grown under different conditions using a reverse transcription-coupled PCR (RT-PCR) assay. D. radiodurans was grown in TGY medium (0.8% w/v tryptone, 0.1% w/v glucose and 0.4% w/v yeast extract) at 30°C, 200 rpm and samples removed hourly for turbidity analysis at 600 nm. Cultures (100 ml) were grown to early log phase (A600 = 0.1) and well into stationary phase (40 h after inoculation). Oxidised log phase cells were prepared by incubating early log phase cells with 10 mM H2O2 for 1 h. Superoxide-treated log phase cells were prepared by incubating early log phase cells with 10 mM menadione for 1 h. Rehydrated log phase cells were prepared by harvesting early log phase cells by centrifugation (2000 g for 10 min) and then lyophilising. Several days later the cells were resuspended in TGY medium and incubated at 30°C, 200 rpm for 2 h before processing for RT-PCR. Slowly dehydrated, rehydrated log phase cells were prepared by harvesting early log phase cells by centrifugation (including a wash with PBS) and then resuspending the pellets in 1 ml PBS. The resuspended pellets were placed into 35 mm petri-dishes and then sealed in a desiccator over silica-gel at 30°C for 58 days. Cells were resuspended in TGY medium and incubated at 30°C, 200 rpm for 2 hours before processing for RT-PCR. Cultures were prepared in triplicate for each set of conditions.
RNA was extracted from the cell pellets using TRIzol reagent according to the manufacturers instructions, dissolved in ddH2O and adjusted to 125 μg/ml (from A260). The RNA was then further treated with DNase I according to the manufacturers recommendations in order to remove traces of genomic DNA. Reverse transcription was performed in a 10 μl reaction containing 10 pmol specific-downstream primer (5' AACGCTAGCGGTCCTTGACCACCG 3'), 0.5 μg DNase-treated RNA, 500 μM of each dNTP and 100 U M-MLV reverse transcriptase. Reactions were incubated at 50°C for 1 h before being terminated by the addition of 40 μl TE buffer and storage at -20°C. Control RT reactions were also performed which contained all of the reaction components except the specific-downstream primer.
Polymerase Chain Reaction (PCR) was performed in 20 μl assays and contained 1.0 U Pfu polymerase, 200 μM of each dNTP, 10 pmol each of upstream primer (5' ACCAGCATGGGGCGGCGTGATCTG 3') and downstream primer (5' TCCCAGCCCTTGAAGGCATAGAAG 3'), 5 μl RT or control-RT reaction and 5% (v/v) DMSO. The PCR cycle was 45 sec at 95°C (dissociation), 45 sec at 60°C, and 75 sec at 72°C (extension) for 40 cycles. All conditions were analysed in a single PCR experiment which contained samples of the triplicate first-strand cDNAs for each of the six growth conditions, individual controls for each of these samples, and a range of empirically determined dilutions of a gene-specific template which demonstrated that amplification was within the exponential range. The gene-specific template was prepared from D. radiodurans genomic DNA by PCR using primers external to those used in the RT-PCR reactions and the product was quantified according to adsorption at 260 nm . The identity of the RT-PCR product was confirmed by its co-migration with the product of the gene-specific template as determined by agarose gel electrophoresis. Data were obtained by densitometry (Syngene GeneGenius) of PCR products resolved using agarose gel electrophoresis and stained with ethidium bromide. Densitometric data for each sample were converted to the equivalent concentration of gene-specific template from a linear calibration plot of log [gene-specific template concentration] versus relative density using GeneTools software (Syngene) .
We are grateful to the Biotechnology and Biological Sciences Research Council for financial support and for the award of a studentship to DIF.
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