Figure 2

Acetaldehyde induced modification and inactivation of BSL-B. A) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of B. subtilis lipase B (BSL-B) treated with acetaldehyde (0-0.5 M) in glycine/NaOH buffer (10 mM, pH 10). S: Molecular weight standard. Arrows indicate putative lipase dimers (~ 40 kDa) and trimers (~ 60 kDa). B) Soluble protein concentration and residual hydrolytic activity of BSL-B after treatment for 24 h with acetaldehyde (0-1.8 M) in glycine/NaOH buffer (10 mM, pH 10). C) Isoelectric focusing (IEF) gel (pH 3-10) of BSL-B after treatment with (0.5 M) and without acetaldehyde in glycine/NaOH (10 mM, pH 10). M: IEF marker. -: BSL-B without acetaldehyde. +: BSL-B with acetaldehyde.