Mechanism of acetaldehyde-induced deactivation of microbial lipases
© Franken et al; licensee BioMed Central Ltd. 2011
Received: 19 July 2010
Accepted: 22 February 2011
Published: 22 February 2011
Microbial lipases represent the most important class of biocatalysts used for a wealth of applications in organic synthesis. An often applied reaction is the lipase-catalyzed transesterification of vinyl esters and alcohols resulting in the formation of acetaldehyde which is known to deactivate microbial lipases, presumably by structural changes caused by initial Schiff-base formation at solvent accessible lysine residues. Previous studies showed that several lipases were sensitive toward acetaldehyde deactivation whereas others were insensitive; however, a general explanation of the acetaldehyde-induced inactivation mechanism is missing.
Based on five microbial lipases from Candida rugosa, Rhizopus oryzae, Pseudomonas fluorescens and Bacillus subtilis we demonstrate that the protonation state of lysine ε-amino groups is decisive for their sensitivity toward acetaldehyde. Analysis of the diverse modification products of Bacillus subtilis lipases in the presence of acetaldehyde revealed several stable products such as α,β-unsaturated polyenals, which result from base and/or amino acid catalyzed aldol condensation of acetaldehyde. Our studies indicate that these products induce the formation of stable Michael-adducts at solvent-accessible amino acids and thus lead to enzyme deactivation. Further, our results indicate Schiff-base formation with acetaldehyde to be involved in crosslinking of lipase molecules.
Differences in stability observed with various commercially available microbial lipases most probably result from different purification procedures carried out by the respective manufacturers. We observed that the pH of the buffer used prior to lyophilization of the enzyme sample is of utmost importance. The mechanism of acetaldehyde-induced deactivation of microbial lipases involves the generation of α,β-unsaturated polyenals from acetaldehyde which subsequently form stable Michael-adducts with the enzymes. Lyophilization of the enzymes from buffer at pH 6.0 can provide an easy and effective way to stabilize lipases toward inactivation by acetaldehyde.
Microbial lipases (triacylglycerol hydrolases, EC 188.8.131.52) belong to the α/β-hydrolase family of enzymes [1, 2] and catalyze the hydrolysis of triglycerides to glycerol and fatty acids [3, 4]. Furthermore, lipases are the most frequently used biocatalysts in organic chemistry due to its ready availability and low cost production, lack of cofactors, broad substrate specificity and high enantioselectivity as well as high stability in non-aqueous media such as ionic liquids, supercritical fluids and organic solvents [5, 6]. Under non-aqueous reaction conditions lipases catalyze the synthesis of esters by esterification, inter-esterification, and transesterification [4, 7, 8].
Lipase catalyzed transesterifications of alcohols with esters are equilibrium reactions that need to be shifted toward the product site. Therefore, several activated esters including enol- and ethoxyvinyl esters have been tested as substrates [9, 10] with enol esters such as isopropenyl acetate or vinyl acetate being the most useful acyl donors . After cleavage, the enol tautomerizes to a carbonyl compound (acetaldehyde or acetone) which can no longer serve as a substrate for the back reaction, thus rendering the system fast and irreversible and also facilitating downstream processing due to the volatility of the carbonyl product [9, 11]. Furthermore, the stoichiometrically generated acetaldehyde can be used in a high-throughput assay to detect transesterification activities of lipases and esterases directly in the organic phase . Despite of these advantages, aldehydes are generally known to act as alkylating reagents by forming Schiff-bases in a Maillard-type reaction, in particular with enzyme lysine ε-amino groups. Although Schiff-base formation as such is a reversible reaction, several follow-up reactions finally render this modification irreversible . Hence, acetaldehyde affects various enzymatic properties as shown for different enzymes of biotechnological significance such as lipases, 2-deoxy-D-ribose 5-phosphate aldolase (DERA), and thiamine-diphosphate dependent enzymes, respectively [14–17]. Acetaldehyde treatment of commercial lipase preparations from Candida rugosa and Galactomyces geotrichum (formerly Geotrichum candidum) resulted in reduced activity and enantioselectivity, whereas only moderate effects were observed with lipases from Pseudomonas sp. or Rhizopus oryzae[18, 19]. C. rugosa lipase was stabilized by immobilization, either covalently on an epoxy-activated carrier resin or by adsorption on Celite 545 [18, 20]. Aldehyde-based deactivation also represents a problem in several lipase-based industrial biotransformations, e.g. in the formation of trans-2-methoxycyclohexanol and 3-(4-methoxyphenyl) glycidic acid . Only recently, the activity of C. rugosa lipase was significantly increased by immobilisation of the enzyme in crosslinked-enzyme aggregates (CLEAS) together with BSA as a "proteic feeder" . Further, activity has been improved by "pH-tuning" of lipases prior to their use in organic solvents, meaning that the enzyme was lyophilized from a buffer with optimal pH for biocatalysis .
Current hypotheses infer that the sensitivity of lipases toward acetaldehyde depends on: (1) the microbial source from which the lipase originates; (2) its molecular weight; (3) the number of lysine residues; (4) their solvent accessibility; (5) a covalent modification of lid-domain residues (for G. geotrichum and C. rugosa lipases); and (6) the pKa-values of the lysine ε-amino groups [19, 24]. The latter hypothesis assumed that the nucleophilicity of lysine ε-amino groups would define their contribution to the deactivation reaction and calculated pKa-values were used to predict Schiff-base formations with lysine ε-amino groups of higher pKa-values and thus increasing acetaldehyde sensitivity of the respective lipase . Comprehensive mutagenesis studies of 2-deoxy-D-ribose 5-phosphate aldolase  and prolipase from R. oryzae failed to achieve complete stabilisation against aldehyde deactivation and furthermore indicated the involvement of several different sites in these enzymes.
In this study, we demonstrate that the protonation state of lysine ε-amino groups in microbial lipases has a significant impact on their sensitivity against acetaldehyde. We also show that α,β-unsaturated polyenals derived from aldol condensation of acetaldehyde in the presence of primary amino compounds or NaOH have a major impact on the deactivation process, most probably by forming stable Michael-adducts with lysine and other side chains.
Results and Discussion
The protonation state determines the sensitivity of lipases toward acetaldehyde
Schiff-base formation depends on the protonation state of primary amino groups, as only a deprotonated amino group (-NH2) can nucleophilically attack the acetaldehyde carbonyl group. Thus, it can be predicted that enzyme deactivation caused by Schiff-base formation should depend on the pH of the respective reaction buffer. If biotransformations are performed in organic solvents with lyophilized or immobilized enzymes, their protonation state is affected by the so-called "pH-memory effect" meaning that lyophilization traps the protonation state of a protein in solution [25, 26]. Different commercial enzyme preparations presumably exhibit different protonation states and additionally, stabilizing additives and/or protein impurities are commonly found resulting in heterogeneities which clearly can influence the deactivation behavior of the respective biocatalyst [27–32]. Therefore, we have adjusted the protonation states of lipases from Candida rugosa (CRL), Rhizopus oryzae (ROL), Pseudomonas fluorescens (PFL), and Bacillus subtilis lipases (BSL A and B) by lyophilizing them from buffers of pH 6 and pH 10, respectively, being far below or equal to the average pKa of the lysine ε-amino groups. The freeze-dried enzymes were then treated with acetaldehyde in toluene and the residual activity was determined. We employed toluene as the solvent for our investigation because it is of significant technical importance and is frequently used in technical lipase processes . It is worth to mention that acetaldehyde-treated lipases were not treated with reducing agents such as sodium cyanoborohydride to stabilize Schiff-bases by converting them into stable secondary amines. Hence, the obtained results can be attributed solely to the formation of stable acetaldehyde-protein adducts resulting from Schiff-bases as precursors.
Acetaldehyde induces cross-links
Formaldehyde-induced modifications of model peptides and insulin demonstrated that a Schiff-base modified lysine residue can form stable intra- and intermolecular cross-links with the side chains of arginine, asparagine, glutamine, histidine, tryptophan, and tyrosine [36, 37]. Further, intramolecular cross-links may result from the reaction of lysine-bound Schiff-bases with neighbouring peptide bonds forming 4-imidazolidinone rings . Investigation of possible cross-linked products was performed using BSL-B as a model enzyme. The enzyme was incubated with different concentrations of acetaldehyde (0-0.5 M) in aqueous solution at pH 10 where BSL-B shows maximum activity and stability . Analysis by SDS-PAGE revealed additional bands with higher molecular weight (~ 40 kDa and ~ 60 kDa) appearing at an acetaldehyde concentration of 0.08 M (Figure 2A). The intensity of these bands increased with increasing acetaldehyde concentrations indicating the formation of BSLB-dimers (~ 40 kDa) and trimers (~ 60 kDa) respectively, presumably caused by intermolecular cross-link formation. Parallel determination of the hydrolytic lipase activity and the soluble protein concentration revealed deactivation already at an acetaldehyde concentration of 0.08 M (Figure 2B). Increasing the acetaldehyde concentration to 0.5 M led to a complete deactivation of BSL-B, whereas considerable protein aggregation could be detected only at concentrations above 1.3 M (Figure 2B). Furthermore, a shift toward higher molecular weight was observed for the monomeric form of BSL-B (Figure 2A) which might be explained by a shift of the isoelectric point (pI) due to acetaldehyde-induced modifications. Alves et al. demonstrated that the electrophoretic mobility of a highly acidic protein could be increased by modifying acidic amino acid residues, resulting in an increased pI. This change of the pI increases the ability of the protein to bind SDS [39, 40]. For BSL-B, acetaldehyde induced modification results in a decrease of the pI (Figure 2C), which in turn probably decreases its ability to bind SDS and thus, explains the observed mobility shift (Figure 2A) . Intramolecular cross-links, which most probably also occur, will potentially not give rise to a defined shift of the electrophoretic mobility, because there a several modification sites present on the protein surface, which prevents the formation of specific crossed-linked products. Separate substitution of each lysine residue in BSL-B against arginine and alanine, respectively, did not result in decreased lipase activity (Additional file 2, Figure S1). Thus, acetaldehyde-induced deactivation is not the consequence of modification of a single "hot-spot" lysine residue as previously suggested for G. geotrichum and C. rugosa lipases .
Acetaldehyde sensitivity of BSL-B depends on enzyme concentration
The deactivation behaviour of BSL-B may be explained by spontaneous aggregate formation, a phenomenon that has already been described for lipases of C. rugosa, P. fluorescens, P. aeruginosa, Staphylococcus aureus, and BSL-A , which is 74% identical to BSL-B .
The formation of active and soluble BSL-B aggregates was analyzed by dynamic light scattering over a time range of 24 h (Additional file 3, Tab. S2). Aggregate formation could be detected only at BSL-B concentrations exceeding 0.1 mg/mL. These results indicate that concentration dependent formation of higher molecular lipase aggregates lead to a decelerated deactivation process, probably due to a decreased accessibility of modifiable amino acid side chains within the aggregates.
Acetaldehyde forms α,β-unsaturated polyenals which modify BSL-B
In order to further analyze the coloured reaction products formed in an aqueous solution containing 500 mM acetaldehyde and 20 mM glycine/NaOH-buffer or 10 mM NaOH, respectively, they were extracted from the aqueous solution and subjected to GC/MS analysis, which clearly revealed the presence of α,β-unsaturated polyenals in aqueous acetaldehyde/NaOH-samples with 2,4-hexadienal (m/z: 96, 95, 67) and 2,4,6-octatrienal (m/z: 122) being the most prominent compounds in the yellow coloured acetaldehyde/NaOH sample (see upper row of Figure 4B). Our results are supported by earlier findings reported in the literature, which also demonstrated the formation of α,β-unsaturated polyenals from acetaldehyde in the presence of bases and primary amino compounds in aqueous solution [47–50]. Specifically, 10 mM glycine in an aqueous 500 mM acetaldehyde solution was shown to catalyze the formation 2,4-hexadienal, 2,4,6-octatrienal, 2,4,6,8-decatetraenal, and longer chained polyenals, which leads to a reddish-brown to black colour . Hence, we assume that the colour formation we have observed is caused by the formation of α,β-unsaturated polyenals and the colour differences result from the different sizes of the respective conjugated π-electron systems formed.
α,β-unsaturated polyenals bind to BSL-B by Michael addition
In comparison to acetaldehyde, α,β-unsaturated polyenals cannot only induce the formation of unstable Schiff-bases with primary amino groups, but are also capable of forming stable Michael-adducts by reaction of their double bond in α,β-position with the nucleophilic side chains of arginine, histidine, lysine and serine [51, 52]. We analyzed differently composed samples (BSA (1 mg/mL), BSL-B (1 mg/mL), glycine (10 mM), NaOH (10 mM), and acetaldehyde (500 mM) in different combinations) to detect protein-bound carbonyl groups which are formed only by Michael-addition of α,β-unsaturated polyenals. The 2,4-dinitrophenylhydrazine (DNPH)-assay revealed the formation of protein-carbonyl compounds only in the presence of NaOH and/or primary amino compounds (glycine or protein). In the presence of glycine, a three- to five-fold higher number of carbonyl groups was detected indicating a high catalytic efficiency of glycine as aldol condensation catalyst. The lower values determined for BSL-B as compared to BSA can be attributed to a lower number of modifiable amino acid residues (60 lysine residues per BSA molecule versus 10 per BSL-B molecule). A more precise localization of the modification sites was attempted by analysis of acetaldehyde-treated BSL-B with MALDI-TOF-MS. MS-spectra showed considerable heterogeneity and sufficient material for analyses was difficult to obtain due to the modification of predominantly lysine residues which hampered trypsin digestion during sample preparation. Nevertheless, we succeeded to identify a modified peptide fragment with Mw = 1507.711 Dalton consisting of amino acids Asn26-Lys37. Michael-addition of crotonaldehyde to the native Asn26-Lys37 fragment (Mw = 1437.712 Da) would result in a theoretical molecular mass shift of 70.09 Da to give 1507.802 Da. This result is the first indication that α,β-unsaturated polyenals can interact with BSL-B and form stable Michael-adducts with surface exposed amino acid residues. Further indications were obtained by studying the direct inactivation of BSL-B with 2,4-hexadienal (see below), which was among the major α,β-unsaturated polyenals identified in our samples.
2,4-Hexadienal inactivates BSL-B not only by modification of lysine residues
In order to investigate the direct effect of α,β-unsaturated polyenals, we tested the ability of 2,4-hexadienal to inactivate BSL-B at concentrations of 0 mM, 10 mM, 25 mM, and 50 mM. As expected, BSL-B activity decreased with increasing 2,4-hexadienal concentrations. It is interesting to note that incubation of BSL-B with 50 mM 2,4-hexadienal reduced the hydrolytic activity to 19% after 24 h whereas no activity decrease was detected after incubation with the same concentration of acetaldehyde (Figure 2B).
Lipase-catalyzed transesterification reactions of vinyl esters result in the formation of acetaldehyde which is known to deactivate microbial lipases. Until now, deactivation was thought to be caused by structural changes resulting from initial Schiff-base formation at solvent accessible lysine residues of the enzymes [19, 24]. Using BSL-B as a model enzyme we demonstrate that acetaldehyde induced modifications lead to an increase of the enzymes' molecular mass presumably caused by intermolecular cross-linking and a decrease of the isoelectric point, respectively. These stable modifications of the enzyme can be explained by Michael-addition of α,β-unsaturated polyenals, formed by aldol condensation of acetaldehyde in the presence of bases and/or amino acids [49–52]. Moreover, we found indications that these polyenals act as non-competitive inhibitors of the enzyme.
Our results further demonstrated that acetaldehyde-induced inactivation of microbial lipases can be avoided by adjusting the protonation state of the enzymes. Thus, "pH-tuning" of lipases cannot only preserve activity but also stability of the enzymes by effectively shielding surface exposed amino groups through protonation. This pre-treatment can avoid the formation of α,β-unsaturated polyenals (if no other bases are present) and protonates primary amino groups which are, besides other nucleophilic side chains, most accessible to Michael-addition. As pH-changes may affect the activity and stability of the enzymes, a prerequisite of pH-tuning is sufficient stability and activity at weak acidic to neutral pH. Besides, free amino acids such as glycine, which is frequently used as a buffer component for lipase preparations, should be avoided for lipase preparations used in transesterification reactions with vinyl esters as they amplify the inactivation process.
The following chemicals were of analytical grade: acetaldehyde (Fluka, Taufkirchen, Germany), citric acid monohydrate (Carl Roth, Karlsruhe, Germany), glycine (Carl Roth, Karlsruhe, Germany), KH2PO4 (Carl Roth, Karlsruhe, Germany), sodium dodecyl sulfate (Carl Roth, Karlsruhe, Germany), NaOH (Carl Roth, Karlsruhe, Germany), Na2HPO4 (Carl Roth, Karlsruhe, Germany), and TCA (Carl Roth, Karlsruhe, Germany).
All other chemicals used in this study were of at least technical grade.
Enzymes and other proteins
Samples of lyophilized lipases (triacylglycerol hydrolases, EC 184.108.40.206) from C. rugosa (CRL, Sigma type VII), R. oryzae (ROL, Amano F-AP15), and Pseudomonas fluorescens (PFL, Amano AK) were purchased from Sigma-Aldrich (Taufkirchen, Germany). Lyophilized BSA was purchased from Sigma-Aldrich (Taufkirchen, Germany). Lipases A and B from B. subtilis (BSL-A and BSL-B) were produced and purified as described previously . Briefly, for large-scale production of BSL-A and BSL-B, high cell density fed-batch cultivation using E. coli BL21(DE3) as a host, carrying pET19b derived expression plasmids were performed in an Infors fermenter (30 L) [53–55]. After purification by immobilized-metal affinity chromatography (IMAC)  using a Ni-nitrilo-triacetic acid (NTA) superflow column (30 mL, QIAGEN, Hilden, Germany), imidazole was removed by gel filtration chromatography (G-25 column, Amersham Pharmacia Biotech) using glycine/NaOH-buffer (10 mM, pH 10).
Determination of protein concentrations
Protein concentrations were determined with the Bradford method  using bovine serum albumin (BSA) as the standard.
Adjustment of lipase protonation state
Lipases from C. rugosa, R. oryzae, and P. fluorescens were dissolved in citrate/NaOH-buffer (100 mM, pH 6) or Na2CO3/NaHCO3-buffer (100 mM, pH 10) respectively at a final concentration of 10 mg/mL. BSL-A and BSL-B were stored in glycine/NaOH-buffer (10 mM, pH 10) and diluted 1:2 with glycine/NaOH (10 mM, pH 10) and citrate/NaOH-buffer (100 mM, pH 6), respectively to a final concentration of 0.5 mg/mL. Samples were incubated in screw-capped vials for 1 h at - 80°C prior to lyophilization carried out overnight at 0.011 mbar. In order to avoid pH-shifts due to the change of temperature during freezing, buffers with a small delta pKa/dT were chosen: citrate buffer: dpKa/dT = 0; carbonate buffer: dpKa/dT = -0.009.
Acetaldehyde-induced deactivation under non-aqueous conditions
The lyophilized enzymes were (1) treated with toluene; (2) sonicated for 3 min (Sonorex ultrasonic bath, BANDELIN electronic, Berlin, Germany) to obtain homogenous dispersions in organic solvent; (3) treated with acetaldehyde (0-1 M for BSL-A, BSL-B, and PFL, and 0-2.5 M for CRL and ROL respectively); and (4) incubated for 24 h at room temperature in an overhead mixer (Reax 2 overhead mixer, Heidolph Instruments, Schwabach, Germany). Lipases were extracted from the organic phase with 800 μL Na2HPO4/KH2PO4-buffer (50 mM, pH 8) and the solution subsequently clarified by centrifugation.
Analysis of aldol products of acetaldehyde in the presence of glycine and NaOH
In order to identify the nature of the coloured products, 500 mM acetaldehyde was incubated in the presence of a 20 mM glycine/NaOH buffer and in 10 mM NaOH without glycine. After incubation at 37°C overnight, the reaction products were extracted with dichloromethane and subjected to GC/MS analysis (Siemens, Varian).
Treatment of BSL-B with methylacetimidate
BSL-B was treated with methylacetimidate which results in specific modification of primary amino groups which retain their positive charge and still exhibit high stability at alkaline pH . 2 × 1 mL BSL-B solution (1.0 mg/mL in 10 mM glycine/NaOH-buffer, pH 10) were mixed with 1 mL methylacetimidate solution (0.9 M methylacetimidate in 1 M NaOH, pH 9.5) and incubated for 1 h at room temperature as described previously . An untreated sample without methylacetimidate was used as a control. The glycine/NaOH-buffer in one of the BSL-B samples was substituted with fresh glycine/NaOH-buffer (10 mM, pH 10) using a Sartorius Vivaspin-20 centrifugal concentrator column (4°C, 3000 g) and precipitated proteins were removed by centrifugation. Residual hydrolytic lipase activity was determined with p-nitrophenylpalmitate as the substrate.
Acetaldehyde- and 2,4-hexadienal-induced deactivation of BSL-B
BSL-B (0.025-0.5 mg/mL) dissolved in glycine/NaOH-buffer (10 mM, pH 10) was mixed with acetaldehyde (final concentration: 0-1.8 M), or 2,4-hexadienal (final concentration: 0-50 mM), incubated for 2 h or 24 h at 37°C in a thermo mixer (Eppendorf, Germany) at 300 rpm and the solution clarified by centrifugation.
Acetaldehyde-induced modification of bovine serum albumin
The reaction of acetaldehyde (0-0.5 M) with buffer components and BSA (1 mg/ml) was performed by incubation of samples for 24 h at 37°C in a thermo mixer (Eppendorf, Germany) at 300 rpm and precipitation with trichloroacetic acid (TCA) as described  by mixing samples with 1/10 volume of 70% (w/v) TCA, incubation on ice for 30 min and subsequent centrifugation.
Determination of lipase activity
Lipase indicator plates  were prepared by addition of 50% (v/v) tributyrin and 5% (w/v) gum arabic to molten Luria-Bertani agar medium (500 ml) . Esterase and lipase activity is indicated by the formation of clear halos around the colonies of E. coli BL21(DE3) expressing an active lipolytic enzyme. Lipase activity in solution was measured with p-nitrophenylpalmitate as the substrate as described .
Protein-bound carbonyl groups were detected as previously described  by addition to each sample of 500 μL 2,4-dinitrophenylhydrazine (DNPH) solution (0.1% (w/v) DNPH in 2 M HCl) for 1 h at room temperature with mixing every 15 min (test tube shaker Reax top, Heidolph, Schwabach, Germany). Proteins were precipitated with 500 μL 20% (w/v) TCA by incubation for 15 min on ice and isolated by centrifugation for 30 min. The pellets were washed 3-times with 1 mL 1:1 (v/v) ethyl acetate/ethanol, resuspended in 6 M guanidinium-hydrochloride, 133 mM Tris-HCl buffer containing 13 mM EDTA and the absorption determined at 375 nm.
Each lysine residue of BSL-B was replaced by alanine and arginine, respectively, using the QuikChange® method (Stratagene, ). Plasmid pET19b carrying the B. subtilis lip B gene was amplified using specific mutagenesis oligonucleotide primers (Additional file 4, Tab. S3) and competent E coli DH5α and BL21(DE3) cells were transformed with plasmids containing the mutant genes using a standard procedure .
Protein gel electrophoresis
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed using a 5% stacking and a 12% separating gel . Samples of 20 μg BSL-B were loaded per lane and PageRuler™ prestained protein ladder (Fermentas, St. Leon-Rot, Germany) was used as molecular mass standard. Gels were stained as described .
Isoelectric focusing (IEF)
BSL-B samples in glycine/NaOH-buffer (10 mM, pH 10) were treated with acetaldehyde (0.5 M), incubated overnight at 37°C in a thermomixer (Eppendorf, Germany) at 300 rpm, concentrated using a micro-concentrator column (Centricon-3, Amicon, USA) and loaded on a pre-cast vertical IEF gel (Novex® pH 3-10, Invitrogen, Germany) . The SERVA® liquid mix IEF marker (SERVA® electrophoresis, Germany) was used as the pI standard and gels were stained as described .
Dynamic light scattering (DLS)
Aggregation of BSL-B in glycine/NaOH-buffer (10 mM, pH 10) was analyzed in a temperature controlled Protein Solutions™ DynaPro™ dynamic light scattering instrument (Wyatt Technology Corporation, USA). BSL-B samples (0.1-0.5 mg/mL) were incubated for 24 h at 37°C and the hydrodynamic radii and polydispersities were calculated at frequent intervals using Dynamics V6 software (Wyatt Technology Corporation, USA).
BSL-B (0.5 mg/mL) was dissolved in glycine/NaOH-buffer (10 mM, pH 10), incubated with 0 mM, 50 mM, and 500 mM acetaldehyde for 24 h at 37°C in a thermo mixer (Eppendorf, Germany) at 300 rpm, and analyzed by SDS-PAGE. Coomassie-stained protein bands were cut out and gel slices were incubated 2 times for 10 min in 650 μL 0.1 M ammonium bicarbonate in 30% (v/v) acetonitrile, dried for 20 min in a vacuum centrifuge, rehydrated in 6 μL Tris/HCl-buffer (3 mM, pH 8.8) and digested with 10 ng/μL trypsine (Promega) overnight at room temperature. Peptides were isolated by addition of 5 μL A. bidest., incubation for 15 min at room temperature, addition of 5 μL 0.2% (v/v) trifluoroacetic acid in 30% (v/v) acetonitrile and incubation for 10 min at room temperature. For MALDI-TOF-MS-analysis 0.5 μL of the peptide solution were co-crystallized on a steal plate (Bruker Daltonics) with 0.5 μL matrix (α-cyano-4-hydroxy-trans-cinnamic acid in 50% (v/v) acetonitrile and 0.25% (w/v) trifluoro acetic acid). External calibration was performed by using the Peptide Calibration Standard for mass spectrometry (Bruker Daltonics). The samples were analyzed with an Ultraflex MALDI-TOF/TOF mass spectrometer III (Bruker Daltonics) in positive reflector mode at an acceleration potential of 26.3 kV.
Identification of colored compounds
A solution of 1 mL 10 mM NaOH was incubated with 500 mM acetaldehyde; the coloured compounds were extracted with dichlormethane and analyzed by coupled gas chromatography and mass spectrometry.
Calculation of pKa - and solvent accessibility values
Solvent accessibility and pKa-value calculations were performed with the PROPKA web interface (http://propka.ki.ku.dk/~drogers/)  using all lipase structure coordinates available at the Brookhaven Protein Database (http://www.pdb.org) and a homology based structural model for BSL-B , respectively.
List of abbreviations used
Bacillus subtilis lipase A
Bacillus subtilis lipase B
Candida rugosa lipase
2-deoxy-D-ribose 5-phosphate aldolase
dynamic light scattering
gas chromatography/mass spectrometry
matrix assisted laser desorption/ionization time of flight mass spectrometry
Pseudomonas fluorescens lipase
- p NPP para:
Rhizopus oryzae lipase
sodium dodecyl sulfate polyacrylamide gel electrophoresis
We thank Dipl.-Biol. Tobias Rosenkranz in the group of PD Dr. Jörg Fitter (Institute of Neurosciences and Biophysics, Forschungszentrum Jülich GmbH) for performing DLS measurements. We also thank Dr. Sanjib Kumar Karmee from the Institute of Technical and Macromolecular Chemistry, Aachen University, for the identification of the α,β-unsaturated polyenals by GC/MS. Furthermore, we thank Dr. Sabine Metzger (BMFZ, University of Duesseldorf) and Dr. Melanie Brocker (Institute of Biotechnology I, Forschungszentrum Jülich GmbH) for the analysis of acetaldehyde-modified BSL-B-samples by MALDI-TOF-MS. This work was financed by the Deutsche Forschungsgemeinschaft in frame of the research training group "BioNoCo" GK 1166.
- Nardini M, Dijkstra BW: α/β hydrolase fold enzymes: the family keeps growing. Curr Opin Struct Biol. 1999, 9 (6): 732-737. 10.1016/S0959-440X(99)00037-8.PubMedView ArticleGoogle Scholar
- Ollis DL, Cheah E, Cygler M, Dijkstra BW, Frolow F, Franken SM, Harel M, Remington SJ, Silman I, Schrag J, Sussmann JL, Verschuren KHG, Goldmann A: The α/β hydrolase fold. Protein Eng. 1992, 5 (3): 197-211. 10.1093/protein/5.3.197.PubMedView ArticleGoogle Scholar
- Jaeger KE, Dijkstra BW, Reetz MT: Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases. Annu Rev Microbiol. 1999, 53: 315-351. 10.1146/annurev.micro.53.1.315.PubMedView ArticleGoogle Scholar
- Jaeger KE, Ransac S, Dijkstra BW, Colson C, van Heuvel M, Misset O: Bacterial lipases. FEMS Microbiol Rev. 1994, 15 (1): 29-63. 10.1111/j.1574-6976.1994.tb00121.x.PubMedView ArticleGoogle Scholar
- Gandhi NN, Patil NS, Sawant SB, Joshi JB: Lipase-catalyzed esterification. Catal Rev - Sci Eng. 2000, 42 (4): 439-480. 10.1081/CR-100101953.View ArticleGoogle Scholar
- Jaeger KE, Reetz MT: Microbial lipases form versatile tools for biotechnology. Trends Biotechnol. 1998, 16 (9): 396-403. 10.1016/S0167-7799(98)01195-0.PubMedView ArticleGoogle Scholar
- Krishna SH, Karanth NG: Lipase and lipase-catalyzed esterification reactions in non-aqueous media. Catal Rev - Sci Eng. 2002, 44 (4): 499-591. 10.1081/CR-120015481.View ArticleGoogle Scholar
- Yahya ARM, Anderson WA, Moo-Young M: Ester synthesis in lipase-catalyzed reactions. Enzyme Microb Technol. 1998, 23 (7-8): 438-450. 10.1016/S0141-0229(98)00065-9.View ArticleGoogle Scholar
- Degueil-Castaing M, De Jeso B, Drouillard S, Maillard B: Enzymatic reactions in organic synthesis: 2-ester interchange of vinyl esters. Tetrahedron Lett. 1987, 28 (9): 953-954. 10.1016/S0040-4039(00)95884-2.View ArticleGoogle Scholar
- Kita Y, Takebe Y, Murata K, Naka T, Akai S: Convenient enzymatic resolution of alcohols using highly reactive, nonharmful acyl donors, 1-ethoxyvinyl esters. J Org Chem. 2000, 65 (1): 83-88. 10.1021/jo9910785.PubMedView ArticleGoogle Scholar
- Chenevert R, Pelchat N, Jacques F: Stereoselective enzymatic acylations (transesterifications). Curr Org Chem. 2006, 10: 1067-1094. 10.2174/138527206777698093.View ArticleGoogle Scholar
- Konarzycka-Bessler M, Bornscheuer UT: A high-throughput-screening method for determining the synthetic activity of hydrolases. Angew Chem Int Ed Engl. 2003, 42 (12): 1418-1420. 10.1002/anie.200390365.PubMedView ArticleGoogle Scholar
- Hoffmann T, Meyer RJ, Sorrell MF, Tuma DJ: Reaction of acetaldehyde with proteins: formation of stable fluorescent adducts. Alcohol Clin Exp Res. 1993, 17 (1): 69-74. 10.1111/j.1530-0277.1993.tb00728.x.PubMedView ArticleGoogle Scholar
- Di Lorenzo M, Hidalgo A, Molina R, Hermoso JA, Pirozzi D, Bornscheuer UT: Enhancement of the stability of a prolipase from Rhizopus oryzae toward aldehydes by saturation mutagenesis. Appl Environ Microbiol. 2007, 73 (22): 7291-7299. 10.1128/AEM.01176-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Jennewein S, Schurmann M, Wolberg M, Hilker I, Luiten R, Wubbolts M, Mink D: Directed evolution of an industrial biocatalyst: 2-deoxy-D-ribose-5-phosphate aldolase. Biotechnol J. 2006, 1 (5): 537-548. 10.1002/biot.200600020.PubMedView ArticleGoogle Scholar
- Ledl F, Schleicher E: New aspects of the Maillard reaction in foods and in the human-body. Angew Chem. 1990, 29 (6): 537-548.View ArticleGoogle Scholar
- Mikolajek R, Spiess AC, Pohl M, Lamare S, Büchs J: An activity, stability and selectivity comparison of propioin synthesis by thiamine diphosphate-dependent enzymes in a solid/gas bioreactor. Chembiochem. 2007, 8 (9): 1063-1070. 10.1002/cbic.200700095.PubMedView ArticleGoogle Scholar
- Berger B, Faber K: 'Immunization' of lipase against acetaldehyde emerging in acyl transfer reactions from vinyl acetate. J Chem Soc, Chem Commun. 1991, 1198-1200. 10.1039/c39910001198. 17
- Weber HK, Stecher H, Faber K: Sensitivity of microbial lipases to acetaldehyde formed by acyl-transfer reactions from vinyl esters. Biotechnol Lett. 1995, 17 (8): 803-808. 10.1007/BF00129008.View ArticleGoogle Scholar
- Kaga H, Siegmund B, Neufellner E, Faber K: Stabilization of Candida rugosa Lipase against acetaldehyde by adsorption onto Celite. Biotechnol Tech. 1994, 8 (6): 369-374. 10.1007/BF00154305.View ArticleGoogle Scholar
- Liese A, Seelbach K, Wandrey C: Industrial Biotransformations. 2006, Weinheim: Wiley-VCH, 2View ArticleGoogle Scholar
- Majumder AB, Gupta MN: Stabilization of Candida rugosa lipase during transacetylation with vinyl acetate. Bioresour Technol. 2010, 101: 2877-2879. 10.1016/j.biortech.2009.09.088.PubMedView ArticleGoogle Scholar
- Carrea G, Riva S: Properties and synthetic applications of enzymes in organic solvents. Angew Chem Int Ed. 2000, 39: 2226-2254. 10.1002/1521-3773(20000703)39:13<2226::AID-ANIE2226>3.0.CO;2-L.View ArticleGoogle Scholar
- Weber HK, Zuegg J, Faber K, Pleiss J: Molecular reasons for lipase-sensitivity against acetaldehyde. J Mol Catal B: Enzym. 1997, 3 (1-4): 131-138. 10.1016/S1381-1177(96)00045-8.View ArticleGoogle Scholar
- Zaks A, Klibanov AM: Enzyme-catalyzed processes in organic solvents. Proc Natl Acad Sci USA. 1985, 82 (10): 3192-3196. 10.1073/pnas.82.10.3192.PubMedPubMed CentralView ArticleGoogle Scholar
- Costantino HR, Griebenow K, Langer R, Klibanov AM: On the pH memory of lyophilized compounds containing protein functional groups. Biotechnol Bioeng. 1997, 53 (3): 345-348. 10.1002/(SICI)1097-0290(19970205)53:3<345::AID-BIT14>3.0.CO;2-J.PubMedView ArticleGoogle Scholar
- Bjurlin MA, Bloomer S: Proteolytic activity in commercial triacylglycerol hydrolase preparations. Biocatal Biotrans. 2002, 20 (3): 179-188. 10.1080/10242420290020705.View ArticleGoogle Scholar
- Bjurlin MA, Bloomer S, Haas MJ: Composition and activity of commercial triacylglycerol acylhydrolase preparations. J Am Oil Chem Soc. 2001, 78 (2): 153-160. 10.1007/s11746-001-0236-9.View ArticleGoogle Scholar
- Bjurlin MA, Bloomer S, Haas MJ: Identification of carboxylesterase activities of commercial triacylglycerol hydrolase (lipase) preparations. Eur J Lipid Sci Technol. 2002, 104: 143-155. 10.1002/1438-9312(200203)104:3<143::AID-EJLT143>3.0.CO;2-N.View ArticleGoogle Scholar
- Wells A: What Is in a Biocatalyst?. Org Process Res Dev. 2006, 10: 678-681. 10.1021/op060006u.View ArticleGoogle Scholar
- Yamane T: Biocatalyses in microaqueous organic media. Handbook of Industrial Biocatalysis. Edited by: Hou CT. 2005, CRC Press, 1Google Scholar
- Dominguez de María P, Gago JVS: Causes of unreproducibility of C. rugosa lipase-catalyzed reactions in slightly hydrated organic media. Tetrahedron. 1999, 55 (28): 8555-8566.View ArticleGoogle Scholar
- Eggert T, van Pouderoyen G, Dijkstra BW, Jaeger KE: Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure. FEBS Lett. 2001, 502 (3): 89-92. 10.1016/S0014-5793(01)02665-5.PubMedView ArticleGoogle Scholar
- van Pouderoyen G, Eggert T, Jaeger KE, Dijkstra BW: The crystal structure of Bacillus subtilis lipase: a minimal α/β hydrolase fold enzyme. J Mol Biol. 2001, 309 (1): 215-226. 10.1006/jmbi.2001.4659.PubMedView ArticleGoogle Scholar
- Li H, Robertson AD, Jensen JH: Very fast empirical prediction and interpretation of protein pKa values. Proteins: Struct, Funct, Bioinf. 2005, 61 (4): 704-721. 10.1002/prot.20660.View ArticleGoogle Scholar
- Metz B, Kersten GF, Hoogerhout P, Brugghe H, Timmermans H, de Jong A, Meiring H, ten Hove J, Hennink WE, Crommelin DJ, Jiskoot W: Identification of formaldehyde-induced modifications in proteins: reactions with model peptides. J Biol Chem. 2004, 297: 6235-6234.Google Scholar
- Metz B, Kersten GFA, Hoogerhout P, Brugghe HF, Timmermans HAM, de Jong A, Meiring H, ten Hove J, Hennink WE, Crommelin DJA, Jiskoot W: Identification of formaldehyde-induced modifications in proteins: reactions with model peptides. J Biol Chem. 2004, 279 (8): 6235-6243. 10.1074/jbc.M310752200.PubMedView ArticleGoogle Scholar
- Eggert T, Pencreac'h G, Douchet I, Verger R, Jaeger KE: A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase. Eur J Biochem. 2000, 267 (21): 6459-6469. 10.1046/j.1432-1327.2000.01736.x.PubMedView ArticleGoogle Scholar
- Alves VS, Pimenta DC, Sattlegger E, Castilho BA: Biophysical characterization of Gir2, a highly acidic protein of Saccharomyces cerevisiae with anomalous electrophoretic behavior. Biochem Biophys Res Commun. 2004, 314 (1): 229-234. 10.1016/j.bbrc.2003.12.086.PubMedView ArticleGoogle Scholar
- Garcia-Ortega L, Rios VD, Martinez-Ruiz A, Onaderra M, Lacadena J, del Pozo AM, Gavilanes JD: Anomalous electrophoretic behavior of a very acidic protein: Ribonuclease U2. Electrophoresis. 2005, 26 (18): 3407-3413. 10.1002/elps.200500261.PubMedView ArticleGoogle Scholar
- Lozano P, De Diego T, Gmouh S, Vaultier M, Iborra JL: Criteria to design green enzymatic processes in ionic liquid/supercritical carbon dioxide systems. Biotechnol Prog. 2004, 20: 661-669. 10.1021/bp0342497.PubMedView ArticleGoogle Scholar
- Liou YC, Marangoni AG, Yada RY: Aggregation behavior of Candida rugosa lipase. Food Res Int. 1998, 31 (3): 243-248. 10.1016/S0963-9969(98)00099-4.View ArticleGoogle Scholar
- Fernández-Lorente G, Palomo JS, Fuentes M, Mateo C, Guisán JM, Fernández-Lafuente R: Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties. Biotechnol Bioeng. 2003, 82 (2): 232-237.PubMedView ArticleGoogle Scholar
- Stuer W, Jaeger KE, Winkler UK: Purification of extracellular lipase from Pseudomonas aeruginosa. J Bacteriol. 1986, 168 (3): 1070-1074.PubMedPubMed CentralGoogle Scholar
- Tyski S, Hryniewicz W, Jeljaszewicz J: Purification and some properties of the staphylococcal extracellular lipase. Biochim Biophys Acta. 1983, 749 (3): 312-317. 10.1016/0167-4838(83)90241-8.PubMedView ArticleGoogle Scholar
- Acharya P, Rao NM: Stability studies on a lipase from Bacillus subtilis in guanidinium chloride. J Protein Chem. 2003, 22 (1): 51-60. 10.1023/A:1023067827678.PubMedView ArticleGoogle Scholar
- Baigrie LM, Cox RA, Slebocka-Tilk H, Tencer M, Tidwell TT: Acid-catalyzed enolization and aldol condensation of acetaldehyde. J Am Chem Soc. 1985, 107 (12): 3640-3645. 10.1021/ja00298a039.View ArticleGoogle Scholar
- Esteve W, Nozière B: Uptake and reaction kinetics of acetone, 2-butanone, 2,4-pentanedione, and acetaldehyde in sulfuric acid solutions. J Phys Chem A. 2005, 109 (48): 10920-10928. 10.1021/jp051199a.PubMedView ArticleGoogle Scholar
- Nozière B, Cordova A: A kinetic and mechanistic study of the amino acid catalyzed aldol condensation of acetaldehyde in aqueous and salt solutions. J Phys Chem A. 2008, 112 (13): 2827-2837.PubMedView ArticleGoogle Scholar
- Nozière B, Dziedzic P, Córdova A: Formation of secondary light-absorbing "fulvic-like" oligomers: A common process in aqueous and ionic atmospheric particles?. Geophys Res Lett. 2007, 34: L21812-View ArticleGoogle Scholar
- Sayre LM, Lin D, Yuan Q, Zhu X, Tang X: Protein adducts generated from products of lipid oxidation: focus on HNE and one. Drug Metab Rev. 2006, 38 (4): 651-675. 10.1080/03602530600959508.PubMedView ArticleGoogle Scholar
- Ichihashi K, Osawa T, Toyokuni S, Uchida K: Endogenous formation of protein adducts with carcinogenic aldehydes: implications for oxidative stress. J Biol Chem. 2001, 276 (26): 23903-23913. 10.1074/jbc.M101947200.PubMedView ArticleGoogle Scholar
- Detry J, Rosenbaum T, Lütz S, Hahn D, Jäger KE, Müller M, Eggert T: Biocatalytic production of enantiopure cyclohexane-trans-1,2-diol using extracellular lipases from Bacillus subtilis. Appl Microbiol Biotechnol. 2006, 72 (6): 1107-1116. 10.1007/s00253-006-0391-9.PubMedView ArticleGoogle Scholar
- Ansorge MB, Kula MR: Investigating expression systems for the stable large-scale production of recombinant L-leucine-dehydrogenase from Bacillus cereus in Escherichia coli. Appl Microbiol Biotechnol. 2000, 53 (6): 668-673. 10.1007/s002539900290.PubMedView ArticleGoogle Scholar
- Korz DJ, Rinas U, Hellmuth K, Sanders EA, Deckwer WD: Simple fed-batch technique for high cell density cultivation of Escherichia coli. J Biotechnol. 1995, 39 (1): 59-65. 10.1016/0168-1656(94)00143-Z.PubMedView ArticleGoogle Scholar
- Hochuli E, Dobeli H, Schacher A: New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J Chromatogr. 1987, 411: 177-184. 10.1016/S0021-9673(00)93969-4.PubMedView ArticleGoogle Scholar
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254. 10.1016/0003-2697(76)90527-3.PubMedView ArticleGoogle Scholar
- Lundblad RL: Chemical reagents for protein modification. 2004, Boca Raton, Florida: CRC Press, 3View ArticleGoogle Scholar
- Tawfik DS: Amidination of lysine side chains. The Protein Protocols Handbook. Edited by: Walker JM. 2002, Totowa, N.J.: Humana Press, 479-480. full_text. 2View ArticleGoogle Scholar
- Bennett TP: Membrane filtration for determining protein in the presence of interfering substances. Nature. 1967, 213 (5081): 1131-1132. 10.1038/2131131a0.PubMedView ArticleGoogle Scholar
- Kok RG, Christoffels VM, Vosman B, Hellingwerf KJ: Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases. J Gen Microbiol. 1993, 139 (10): 2329-2342.PubMedView ArticleGoogle Scholar
- Sambrook J, MacCallum P, Russell D: Molecular Cloning: A Laboratory Manual. 2001, Cold Spring Harbor Laboratory Press, 3Google Scholar
- Winkler UK, Stuckmann M: Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. J Bacteriol. 1979, 138 (3): 663-670.PubMedPubMed CentralGoogle Scholar
- Fields R, Dixon HB: Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine. Biochem J. 1971, 121 (4): 587-589.PubMedPubMed CentralView ArticleGoogle Scholar
- Kunkel TA: Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci USA. 1985, 82 (2): 488-492. 10.1073/pnas.82.2.488.PubMedPubMed CentralView ArticleGoogle Scholar
- Hanahan D: Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983, 166 (4): 557-580. 10.1016/S0022-2836(83)80284-8.PubMedView ArticleGoogle Scholar
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227 (5259): 680-685. 10.1038/227680a0.PubMedView ArticleGoogle Scholar
- Kang D, Gho YS, Shu M, Kang C: Highly sensitive and fast protein detection with Coomassie Brilliant Blue in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bull Korean Chem Soc. 2002, 23 (11): 1511-1512. 10.5012/bkcs.2002.23.11.1511.View ArticleGoogle Scholar
- Righetti P, Drysdale JW: Isoelectric focusing in polyacrylamide gels. Biochim Biophys Acta. 1971, 236 (1): 17-28.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.