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Table 6 Substrate screening of crude extract of E. coli harboring SDR from B. pseudomallei

From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays

Substrate Enzyme activity (nM/min)
NAD+ NADP+
Sugars
 D-Glucose na 158.67 ± 24.36
 D-Galactose na na
 D-Lactose na na
 D-Fructose na na
 D-Xylose na 25.89 ± 14.14
 D-Mannose na na
 Maltose na na
 Sucrose na na
Alcohols and aldehydes
 Methanol na na
 Ethanol 131.31 ± 25.26 na
 1-Propanol 145.60 ± 8.42 na
 1-Butanol na 134.23 ± 16.50
 1-Hexanol na 90.34 ± 4.72
 1-Octanol na 33.11 ± 11.78
 Acetaldehyde na na
 Propionaldehyde na na
 Butyraldehyde na na
  1. na no activity