Table 6 Substrate screening of crude extract of E. coli harboring SDR from B. pseudomallei
From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays
Substrate | Enzyme activity (nM/min) | |
|---|---|---|
NAD+ | NADP+ | |
Sugars | ||
 D-Glucose | na | 158.67 ± 24.36 |
 D-Galactose | na | na |
 D-Lactose | na | na |
 D-Fructose | na | na |
 D-Xylose | na | 25.89 ± 14.14 |
 D-Mannose | na | na |
 Maltose | na | na |
 Sucrose | na | na |
Alcohols and aldehydes | ||
 Methanol | na | na |
 Ethanol | 131.31 ± 25.26 | na |
 1-Propanol | 145.60 ± 8.42 | na |
 1-Butanol | na | 134.23 ± 16.50 |
 1-Hexanol | na | 90.34 ± 4.72 |
 1-Octanol | na | 33.11 ± 11.78 |
 Acetaldehyde | na | na |
 Propionaldehyde | na | na |
 Butyraldehyde | na | na |