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Table 5 Steady-state kinetic parameters of GDH and G6PD determined by colorimetric WST-8 assay compared with previous studies using UV-spectrophotometry

From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays

Enzyme

KM (mM)

Reference

Glucose

NAD+

Bacillus GDH

17.1 ± 2.0

0.271 ± 0.027

The present study

31.8 ± 0.4

0.210 ± 0.001

Chen et al., 2011 [35]

Enzyme

KM (μM)

References

G6P

NADP+

Human G6PD

42.3 ± 4.8

2.1 ± 0.4

The present study

47.8 ± 4.2

7.2 ± 1.8

Boonyuen et al., 2016 [32]

45.8 ± 1.6

4.67 ± 0.32

Huang et al., 2008 [36]

52 ± 4

7.07 ± 1.13

Wang et al., 2005 [37]