From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays
Enzyme | KM (mM) | Reference | |
---|---|---|---|
Glucose | NAD+ | ||
Bacillus GDH | 17.1 ± 2.0 | 0.271 ± 0.027 | The present study |
31.8 ± 0.4 | 0.210 ± 0.001 | Chen et al., 2011 [35] | |
Enzyme | KM (μM) | References | |
G6P | NADP+ | ||
Human G6PD | 42.3 ± 4.8 | 2.1 ± 0.4 | The present study |
47.8 ± 4.2 | 7.2 ± 1.8 | Boonyuen et al., 2016 [32] | |
45.8 ± 1.6 | 4.67 ± 0.32 | Huang et al., 2008 [36] | |
52 ± 4 | 7.07 ± 1.13 | Wang et al., 2005 [37] |