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Table 5 Steady-state kinetic parameters of GDH and G6PD determined by colorimetric WST-8 assay compared with previous studies using UV-spectrophotometry

From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays

Enzyme KM (mM) Reference
Glucose NAD+
Bacillus GDH 17.1 ± 2.0 0.271 ± 0.027 The present study
31.8 ± 0.4 0.210 ± 0.001 Chen et al., 2011 [35]
Enzyme KM (μM) References
G6P NADP+
Human G6PD 42.3 ± 4.8 2.1 ± 0.4 The present study
47.8 ± 4.2 7.2 ± 1.8 Boonyuen et al., 2016 [32]
45.8 ± 1.6 4.67 ± 0.32 Huang et al., 2008 [36]
52 ± 4 7.07 ± 1.13 Wang et al., 2005 [37]