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Table 4 Spike recoveries of GDH and G6PD in FBS using colorimetric WST-8 assay

From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays

Assay

Enzyme activity (nmole/min)

%Recovery

Expected

Measured

GDH

0.071

0.084 ± 0.002

118.78 ± 1.81

G6PD

1.024

1.235 ± 0.004

120.57 ± 0.33