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Table 3 GDH and G6PD activities measured by colorimetric WST-8 and UV spectrophotometry methods

From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays

Method Enzyme activity (nmole/min/μg)
Purified GDH Purified G6PD G6PD in crude extract
Colorimetric WST-8 (450 nm) 0.069 ± 0.001 12.51 ± 0.36 0.112 ± 0.001
UV-spectrophotometry (340 nm) 0.078 ± 0.004 14.88 ± 0.39 0.145 ± 0.003