Table 3 GDH and G6PD activities measured by colorimetric WST-8 and UV spectrophotometry methods
From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays
Method | Enzyme activity (nmole/min/μg) | ||
|---|---|---|---|
Purified GDH | Purified G6PD | G6PD in crude extract | |
Colorimetric WST-8 (450 nm) | 0.069 ± 0.001 | 12.51 ± 0.36 | 0.112 ± 0.001 |
UV-spectrophotometry (340 nm) | 0.078 ± 0.004 | 14.88 ± 0.39 | 0.145 ± 0.003 |