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Table 2 Accuracy and precision for measurement of NAD(P)H by colorimetric WST-8 and UV spectrophotometry methods

From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays

Method   Prepared amount (nmole) Measured amount (nmole) Accuracy (%RE) Precision (%CV) Z’- factor
Within-run Between-run
Colorimetric WST-8 (450 nm) NADH 4.3 4.33 1.9 1.8 1.7 0.93
8.5 8.44 −0.7 1.3 1.0 0.95
12.8 12.44 −2.5 0.6 0.9 0.98
NADPH 4.3 4.39 2.1 2.2 4.5 0.90
8.6 8.72 1.4 0.8 1.7 0.99
12.9 12.98 0.6 1.5 2.1 0.98
UV-spectrophotometry (340 nm) NADH 102 101.70 −0.3 0.7 1.2 0.97
212.5 208.61 −1.8 1.1 1.2 0.96
340 329.34 −3.1 1.1 1.0 0.96
NADPH 103.2 105.50 2.2 1.0 1.1 0.96
215 215.88 0.4 1.3 0.8 0.96
344 344.91 0.3 0.5 0.9 0.98