Table 2 Accuracy and precision for measurement of NAD(P)H by colorimetric WST-8 and UV spectrophotometry methods
From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays
Method |  | Prepared amount (nmole) | Measured amount (nmole) | Accuracy (%RE) | Precision (%CV) | Z’- factor | |
|---|---|---|---|---|---|---|---|
Within-run | Between-run | ||||||
Colorimetric WST-8 (450 nm) | NADH | 4.3 | 4.33 | 1.9 | 1.8 | 1.7 | 0.93 |
8.5 | 8.44 | −0.7 | 1.3 | 1.0 | 0.95 | ||
12.8 | 12.44 | −2.5 | 0.6 | 0.9 | 0.98 | ||
NADPH | 4.3 | 4.39 | 2.1 | 2.2 | 4.5 | 0.90 | |
8.6 | 8.72 | 1.4 | 0.8 | 1.7 | 0.99 | ||
12.9 | 12.98 | 0.6 | 1.5 | 2.1 | 0.98 | ||
UV-spectrophotometry (340 nm) | NADH | 102 | 101.70 | −0.3 | 0.7 | 1.2 | 0.97 |
212.5 | 208.61 | −1.8 | 1.1 | 1.2 | 0.96 | ||
340 | 329.34 | −3.1 | 1.1 | 1.0 | 0.96 | ||
NADPH | 103.2 | 105.50 | 2.2 | 1.0 | 1.1 | 0.96 | |
215 | 215.88 | 0.4 | 1.3 | 0.8 | 0.96 | ||
344 | 344.91 | 0.3 | 0.5 | 0.9 | 0.98 | ||