Table 1 The limit of NAD(P)H detection of colorimetric WST-8 and UV spectrophotometric methods
From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays
Method | Â | LOD (nmole) | LLOQ (nmole) | ULOQ (nmole) |
|---|---|---|---|---|
Colorimetric WST-8 (450 nm) | NADH | 0.32 | 0.4 | 19 |
NADPH | 0.29 | 0.4 | 19 | |
UV spectrophotometry (340 nm) | NADH | 1.65 | 3.41 | 400 |
NADPH | 1.72 | 4.11 | 400 |