Skip to main content

Table 1 The limit of NAD(P)H detection of colorimetric WST-8 and UV spectrophotometric methods

From: Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays

Method   LOD (nmole) LLOQ (nmole) ULOQ (nmole)
Colorimetric WST-8 (450 nm) NADH 0.32 0.4 19
NADPH 0.29 0.4 19
UV spectrophotometry (340 nm) NADH 1.65 3.41 400
NADPH 1.72 4.11 400