Fig. 1

a - k: Expression profile analysis depending on the treatment with Ionomycin. Four different groups of mRNA are produced from PaTu 8988t lysates: 1) Untreated control lysates, 2) Lysates from cells treated with Ionomycin 1 h previously, 3) Cell lysates in which Sp1 is repressed with RNA interference, and 4) Lysates stimulated with siRNA for Sp1 and additionally with Ionomycin for 1 h. The lysates were assessed in the expression profile analysis that contained 89 different genes involved in the carcinogenesis of pancreatic cancer. After the administration of Ionomycin, altogether 11 genes (Fig. 1a - k) were expressed. The basal activity of the genes in the control lysate is normalized to the value of 100, and the influence of the regulatory change is shown as the x-fold increase or decrease of this control. Data are presented as mean ± SD. The t-test was used for statistical evaluation of the data. P values of <0.05 were considered statistically significant