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Table 2 Phosphorylation of Ypd1-G68X and phosphorelay activity

From: Role of the highly conserved G68 residue in the yeast phosphorelay protein Ypd1: implications for interactions between histidine phosphotransfer (HPt) and response regulator proteins

Protein aPhosphorylation (%*) bPhosphorelay (%*)
WT 100 ± 0 100 ± 0
G68S 94 ± 3.6 81 ± 18
G68A 82 ± 16 80 ± 8.0
G68V 2.5 ± 3.5 0.36 ± 0.6
G68L 0.4 ± 0.6 0.28 ± 0.5
G68E 0.3 ± 0.5 0.08 ± 0.15
G68Q 40 ± 8.3 0.9 ± 1.6
  1. *Values are expressed as a percentage of wild-type. Standard deviations were calculated based on three replicates under similar conditions
  2. aPhosphorylation of Ypd1 mutants from Sln1-R1 as phosphondonor
  3. bPhosphorelay from Sln1-R1 to Ssk1-R2