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Fig. 2 | BMC Biochemistry

Fig. 2

From: Role of the highly conserved G68 residue in the yeast phosphorelay protein Ypd1: implications for interactions between histidine phosphotransfer (HPt) and response regulator proteins

Fig. 2

Ability of Ypd1 to accept phosphoryl groups is affected by nature of residue in the H + 4 position. Phosphoryl transfer reactions contained equimolar concentrations of Sln1-R1 and Ypd1. Reaction was quenched at 5 min with stop buffer containing EDTA and separated by SDS-PAGE. The gel bands were detected by phosphorimaging and analyzed using ImageJ software. The amount of radiolabel in Ypd1-G68X mutants was quantified and compared with the amount of radiolabel seen for the band corresponding to wild-type YPD1 (normalized to 100%)

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BMC Biochemistry

ISSN: 1471-2091

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