Fig. 3
From: Up-regulation of DcR3 in microbial toxins-stimulated HUVECs involves NF-κB signalling
Toll-like receptor expression in HUVECs and Jurkat cells. TLR-2 and TLR-4 receptor expression was assayed using flow cytometry. Expression of both TLR-2 and TLR-4 was in HUVECs; however, TLR-4 expression was significantly higher than TLR-2 expression in Jurkat cells. a Jurkat cells were assayed by flow cytometry without antibody incubation. b, c Jurkat cells were treated by PE-labbled TLR-2 primary antibody and FITC-labbled TLR-4 primary antibody separately and then assayed the TLRs expression by flow cytometry. d HUVECs were assayed by flow cytometry without antibody incubation. e, f HUVECs cells were treated by PE-labbled TLR-2 primary antibody and FITC-labbled TLR-4 primary antibody separately and then assayed the TLRs expression by flow cytometry. g The TLR-2 and TLR-4 expression ratio in Jurkat cells and HUVECs. h The TLR-2 and TLR-4 fluorescence intensity in Jurkat cells and HUVECs