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Fig. 1 | BMC Biochemistry

Fig. 1

From: Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host

Fig. 1

Amino acid sequences of Ae. aegypti midgut zymogen (no leader) proteases. In order to improve solubility of recombinant proteases, the leader (signal) sequence was removed to produce the no leader zymogen as shown. Since the genes were cloned into the pET28a vector (utilizing the NdeI restriction site, CATATG), the resulting recombinant proteases will contain an N-terminal Methionine (shown in red), as well as the his6-tag linker (MGSSHHHHHHSSGLVPRGSH) upstream of the Met group (shown in red). The arrow points to the propeptide cleavage site required to activate the zymogen to the active mature protease

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BMC Biochemistry

ISSN: 1471-2091

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