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Fig. 2 | BMC Biochemistry

Fig. 2

From: Purification and characterization of cysteine protease from miswak Salvadora persica

Fig. 2

a Chromatography of miswak protease P1 DEAE-Sepharose fraction on Sephacryl S-200 column. (•___•) Absorbant at 280 nm, (x ___ x) units/fraction. b Molecular weight value for miswak protease P1 was calculated from calibration curve of Sephacryl S-200 column. Standard proteins from 1 to 5 were 1) Cytochrome C (12,400 Da); 2) Carbonic anhydrase (29,000 Da); 3)Bovine albumin (66,000 Da); 4)Alcohol dehydrogenase (150,000 Da); 5) β-Amylase (200,000 Da). Void volume was determined with Dextran blue (2000, 000 Da)

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