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Table 1 Proteins Detected in Untreated (Control) and IAPP Treated Rin-5F Cells. Data were obtained from three independent experiments, where each experiment was repeated three times. Data is only reported for proteins detected in all three repeats. F1-F12 are the 12 fractions from OFFGEL™ electrophoresis, separated by pI. Complete data is in Additional file 1: Table S1

From: Effect of IAPP on the proteome of cultured Rin-5F cells

Property F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12
Theoretical pH range <  3.6 3.6–4.2 4.2–4.7 4.7–5.3 5.3–5.9 5.9–6.5 6.5–7.1 7.1–7.6 7.6–8.2 8.2–8.8 8.8–9.2 >  9.2
# proteins control Ex1 29 48 48 16 9 4 53 92 54 26 20 19
Mean pI control Ex1 4.8 4.7 4.9 5.2 5.3 5.8 6.6 7.2 7.6 8.2 8.3 8.6
Mean % protein coverage control Ex1 29.8 32.9 35.9 26.1 31.3 26.6 24.7 26.2 29.5 24.7 27.2 28.1
# proteins IAPP Ex1 33 46 48 53 62 60 70 46 59 15 27 4
Mean pI IAPP Ex1 5.0 4.8 4.9 5.1 5.6 6.2 6.4 7.1 7.5 8.2 8.5 8.8
Mean % protein coverage IAPP Ex1 27.9 33.7 29.6 29.1 25.3 28.1 26.6 25.4 27.4 18.8 31.2 27.4
# proteins control Ex2 106 189 114 180 190 151 214 238 155 192 169 236
Mean pI control Ex2 4.9 4.9 5.1 5.4 6.0 6.4 6.8 7.0 7.6 8.0 8.1 8.5
Mean % protein coverage control Ex2 23.2 29.5 31.8 30.1 29.1 24.2 24.7 24.9 29.5 24.5 26 25.9
# proteins IAPP Ex2 46 131 149 154 152 223 264 270 223 219 145 187
Mean pI IAPP Ex2 4.8 4.8 5.1 5.3 6.0 6.6 6.7 7.1 7.7 8.0 8.6 8.9
Mean % protein coverage IAPP Ex2 21.6 24.3 22.9 26.2 24.5 24.6 24.5 24.2 25.2 24.4 20.7 25.7
# proteins control Ex3 5 57 52 60 45 64 69 75 30 41 46 0
Mean pI control Ex3 4.7 4.8 5.1 5.3 5.9 6.5 6.6 7.2 7.8 7.8 8.5
Mean % protein coverage control Ex3 24.2 32.9 29.9 23.7 22.8 26.2 25.3 25.6 27 30.8 31.4
# proteins IAPP Ex3 15 33 38 56 50 56 74 53 44 58 27 9
Mean pI IAPP Ex3 5.0 4.8 4.9 5.3 6.0 6.6 6.5 7.0 7.8 7.9 8.4 8.9
Mean % protein coverage IAPP Ex3 25.2 34.8 28.1 30.8 23.7 27.5 23.8 26.3 27.9 32.4 33.5 31.7