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Table 5 The steps of the procedure used for assessing the activity of catalase

From: Simple spectrophotometric assay for measuring catalase activity in biological tissues

Reagents

Test

Standard

Blank

Catalase source sample

500 μl

Distilled water

500 μl

1500 μl

Hydrogen peroxide

1000 μl

1000 μl

The tubes were mixed with a vortex and incubated at 37 °C for 2 min, after which the following substance was added:

Working solution

6000 μl

6000 μl

6000 μl

Next, the tubes were vortexed for 5 s and then kept at room temperature for 10 min in the dark. The changes in absorbance were recorded at 440 nm against the reagent blank