From: Simple spectrophotometric assay for measuring catalase activity in biological tissues
Reagents | Test | Standard | Blank |
---|---|---|---|
Catalase source sample | 500 μl | – | – |
Distilled water | – | 500 μl | 1500 μl |
Hydrogen peroxide | 1000 μl | 1000 μl | – |
The tubes were mixed with a vortex and incubated at 37 °C for 2 min, after which the following substance was added: | |||
Working solution | 6000 μl | 6000 μl | 6000 μl |
Next, the tubes were vortexed for 5 s and then kept at room temperature for 10 min in the dark. The changes in absorbance were recorded at 440 nm against the reagent blank |