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Table 5 The steps of the procedure used for assessing the activity of catalase

From: Simple spectrophotometric assay for measuring catalase activity in biological tissues

Reagents Test Standard Blank
Catalase source sample 500 μl
Distilled water 500 μl 1500 μl
Hydrogen peroxide 1000 μl 1000 μl
The tubes were mixed with a vortex and incubated at 37 °C for 2 min, after which the following substance was added:
Working solution 6000 μl 6000 μl 6000 μl
Next, the tubes were vortexed for 5 s and then kept at room temperature for 10 min in the dark. The changes in absorbance were recorded at 440 nm against the reagent blank