Isolation of quercetin and mandelic acid from Aesculus indica fruit and their biological activities

BMC Biochemistry

Table 2 Percent AChE and BChE inhibition potentials of Aesculus indica fruit Crude extract and their sub fractions

Sample	Concentration (μg/ml)	Percent AChE (mean ± SEM)	AChE IC₅₀ (μg/ml)	Percent BChE (mean ± SEM)	BChE IC₅₀ (μg/ml)
Crude	1000	84.32 ± 1.22^***		80.42 ± 0.62^***
	500	71.23 ± 1.40^***	145	68.48 ± 3.13^***	200
	250	58.07 ± 1.20^***		52.54 ± 1.26^***
	125	49.37 ± 0.02^***		45.70 ± 0.52^***
Chloroform	1000	86.21 ± 0.49^***		82.56 ± 0.70^***
	500	74.24 ± 0.81^***	85	70.62 ± 0.80^***	160
	250	64.22 ± 0.67^***		58.011 ± 2.68^***
	125	53.82 ± 0.41^***		48.05 ± 0.99^***
Ethyl acetate	1000	75.23 ± 0.55^***	190	69.12 ± 2.60^***	210
	500	63.91 ± 0.84^***		60.63 ± 1.77^***
	250	54.53 ± 0.81^***		51.06 ± 0.37^**
	125	40.61 ± 2.30^***		37.80 ± 0.60^***
Aqueous	1000	71.46 ± 0.71^***		68.87 ± 1.90^***
	500	58.10 ± 1.36^***	350	54.18 ± 2.80^***	400
	250	45.14 ± 0.67^***		38.60 ± 3.46^***
	125	32.71 ± 0.76^***		26.11 ± 0.42^***
n-Hexane	1000	54.15 ± 0.52^***		52.76 ± 1.64^***
	500	44.28 ± 2.21^***	760	40.56 ± 0.88^***	870
	250	34.04 ± 0.91^***		28.49 ± 2.24^***
	125	25.23 ± 0.88^***		21.39 ± 0.69^***
Galanthamine	1000	95.32 ± 0.88		92.50 ± 0.71
	500	84.31 ± 0.55	48	80.66 ± 1.20	66
	250	72.31 ± 0.60		72.72 ± 0.72
	125	64.38 ± 0.54		60.83 ± 0.69

Galanthamine was used as a positive control. Data is represented as (mean ± S.E.M) n = 3. Values significantly different as compared to positive control, ^** P < 0.01; ^*** P < 0.001

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