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Table 1 Percent DPPH and ABTS radical scavenging potential of crude extract and their sub fractions of Aesculus indica fruit using ascorbic acid as standard

From: Isolation of quercetin and mandelic acid from Aesculus indica fruit and their biological activities

Samples

Concentrations (μg/mL)

ABTS percent inhibition (mean ± S.E.M)

ABTS IC50 (μg/mL)

DPPH percent inhibition (mean ± S.E.M)

DPPH IC50 (μg/mL)

Crude

1000

85.58 ± 1.31 ns

 

90.28 ± 0.50 ns

 

500

72.26 ± 1.18***

 

79.21 ± 0.48***

 

250

68.59 ± 1.00***

105

70.01 ± 0.80***

60

125

54.93 ± 1.49***

 

58.23 ± 1.13***

 

62.5

42.66 ± 2.25***

 

50.45 ± 0.52***

 

Chloroform

1000

83.39 ± 0.46*

 

84.27 ± 0.54***

 

500

72.58 ± 0.54***

 

73.66 ± 1.79***

 

250

63.30 ± 0.67***

65

64.56 ± 2.23***

50

125

56.25. ± 2.14***

 

59.24 ± 0.69***

 

62.5

51.83 ± 0.84***

 

47.36 ± 0.76***

 

Ethyl acetate

1000

78.31 ± 0.53***

 

79.04 ± 0.72***

 

500

66.99 ± 0.79***

183

68.77 ± 1.61***

 

250

56.29 ± 0.75***

 

57.33 ± 0.91***

155

125

44.93 ± 0.88***

 

46.70 ± 1.22***

 

62.5

39.49 ± 0.63***

 

40.73 ± 0.37***

 

Aqueous

1000

70.50 ± 0.74***

 

73.82 ± 1.20***

 

500

60.10 ± 2.29***

325

62.62 ± 1.16***

 

250

44.84 ± 2.67***

 

49.47. ± 0.58***

250

125

35.76 ± 0.52***

 

36.56 ± 1.25***

 

62.5

20.66 ± 3.08***

 

26.16 ± 2.36***

 

n-hexane

1000

58.13 ± 0.49***

 

57.03 ± 2.00***

 

500

43.17 ± 0.66***

 

44.44 ± 2.00***

 

250

31.01 ± 2.34***

760

35.22 ± 1.06***

725

125

22.02 ± 1.11***

 

24.29 ± 0.54***

 

62.5

14.19 ± 0.55***

 

15.19 ± 0.50***

 

Ascorbic acid

1000

89.94 ± 0.86

 

94.35 ± 0.7

 

500

86.29 ± 0.79

 

87.90 ± 0.96

 

250

77.32 ± 2.23

23

81.78 ± 2.16

15

125

69.97 ± 3.26

 

73.61 ± 0.85

 

62.5

61.28 ± 1.31

 

63.31 ± 0.76

 
  1. Ascorbic acid was used as a positive control. Data is represented as mean ± S.E.M; (n = 3). Values significantly different as compared to positive control,* P < 0.05; *** P < 0.001, ns: P > 0.05