Identification and characterization of smallest pore-forming protein in the cell wall of pathogenic Corynebacterium urealyticum DSM 7109

Table 6 Zero-current membrane potentials, V_m, of Diph-PC/n-decane membranes in the presence of the channel-forming protein of C. urealyticum, PorACur, measured for a 5-fold gradient of different salts

Electrolyte	Permeability ratios P_cation/P_anion	V_m (mV)^a
KCl	2.5	12 ± 2
LiCl	0.57	−8.2 ± 0.2
KCH₃COO (pH 7)	6.9	23. ± 2

^aThe potential V_m, measured for a fivefold gradient of different salts, is defined as the difference between the potential on the dilute side minus the potential on the concentrated side. The aqueous salt solutions were buffered with 10 mM Tris-HCl (pH 7), and the temperature was 20 °C. The cation/anion permeability ratio was calculated from at least 3 individual experiments [44]

ISSN: 1471-2091