|
Oligonucleotides
|
Sequence 5’➝ 3’
|
|---|
|
Fwd_1714_XbaI
|
GTGTCTAGAGACCTACACTCTAGGAGTTTC
|
|
RP pX cur_1714 KpnI
|
CTGGTACCTTAGAAGCCGAATGCCTG
|
|
RP-cur1714-KpnI pXHis
|
GCTTAAAGGTACCGAAGCCGAATG
|
|
RevpXMJ19
|
CAGACCGCTTCTGCGTTCTG
|
|
Fwd Gst 1714 BamHI
|
CTGAGGATCCGGTAACGCAAC
|
|
Rev Gst 1714 EcoRI
|
GACGAATTCTTAGAAGCCGAATGC
|
|
Fwd Gst 1715BamHI
|
CAGTGGATCCAACGTCGACATG
|
|
Rev Gst 1715 EcoRI
|
CTAGAATTCCTACTTGTTCTCGG
|
|
Fwd GST Seq
|
CACTCCCGTTCTGGATAATG
|
|
Rev GST Seq
|
CACTCCGCTATCGCTACGTGAC
|
|
T7 Promotor
|
TAATACGACTCACTATAGGG
|
|
M13 reverse
|
CAGGAAACAGCTATGAC
|
- Recognition sites for the restriction enzymes are underlined