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Fig. 5 | BMC Biochemistry

Fig. 5

From: Identification and characterization of smallest pore-forming protein in the cell wall of pathogenic Corynebacterium urealyticum DSM 7109

Fig. 5

Study of pore-formation in the presence of pure PorACur of C. urealyticum. a Single-channel recording of a Diph-PC/n-decane membrane in the presence of 10 ng/ml pure PorACur. The aqueous phase contained 1 M KCl and the applied membrane potential was 20 mV; T = 20 °C. b Histogram of the probability P(G) for the occurrence of a given conductivity unit observed with membranes formed of 1% Diph-PC dissolved in n-decane. The histogram was calculated by dividing the number of fluctuations with a given conductance step by the total number of conductance fluctuations in the presence of pure PorACur. Two frequent conductive units were observed for single events taken from different individual membranes. The average conductance of the steps corresponding to the left-side maximum was 1.75 ± 0.25 nS and that of the right-side maximum was 3 nS (65 current steps in total). The aqueous phase contained 1 M KCl, and10 ng/ml protein extract, the applied membrane potential was 20 mV, T = 20 °C

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BMC Biochemistry

ISSN: 1471-2091

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