Fig. 5From: Characterization of association of human mitochondrial lysyl-tRNA synthetase with HIV-1 Pol and tRNA3LysAssociation of the TF-Sx-IN surrogates of Pol with mLysRS. The fusion proteins with spacers made of 2 (S2), 7 (S7), 12 (S12), 17 (S17) or 22 (S22) amino acids were analyzed by SDS-PAGE and their binding affinities for mLysRS were determined in the HTRF assay, as described in Fig. 2. The binding constants and the associated standard deviations result from three independent measurementsBack to article page