Fig. 2From: Feruloyl esterase immobilization in mesoporous silica particles and characterization in hydrolysis and transesterificationEffect of immobilization pH and of enzyme concentration on loading and enzymatic activity. a Effect of immobilization pH on the loading using different buffers. b Effect of enzyme concentration on the loading. c Influence of the immobilization pH and buffer on the BFA/FA molar ratio. The results are compared to those obtained for the free enzyme at pHÂ 7.0 in the respective buffers. d Influence of the immobilization pH on the BFA specific activity. The results are compared to those obtained for the free enzyme at pHÂ 7.0 in the respective buffers. All reactions were in initial velocity. All values are averages of triplicates and the error bars represent one standard deviationBack to article page