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Fig. 6 | BMC Biochemistry

Fig. 6

From: Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain

Fig. 6

Detection of as-APF binding to CKAP4 mutants by LC-MS. Various CKAP4 mutant-immobilized His-tag affinity beads were used to interact with as-APF, and the captured as-APF was analyzed by MS as described in “Materials and Methods”. a The protein staining of SDS-PAGE to show the efficiency of experiment; b The quantitation of as-APF bound to various CKAP4 mutants based on the as-APF standard curve (not shown) also performed with the same LC-MS conditions

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