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Fig. 4 | BMC Biochemistry

Fig. 4

From: Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain

Fig. 4

Characterization of purity and antigenicity of CKAP4 constructs. a SDS-PAGE of normalized amounts of purified CKAP4 constructs followed by silver staining. b–f Western blots using anti-His tag, anti-CKAP4 (G1/296), anti-CKAP4 (epitope region: 216–265), anti-CKAP4 (epitope region: 375–425), and anti-CKAP4 (epitope region: 552–602) primary antibody, respectively. Separate blots were used for each antibody. Molecular weight standards are indicated on left and right

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