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Fig. 1 | BMC Biochemistry

Fig. 1

From: Use of a special Brazilian red-light emitting railroad worm Luciferase in bioassays of NEK7 protein Kinase and Creatine Kinase

Fig. 1

Luciferase assay optimization. a ATP titration: The graph represents the light intensity of each reaction containing different ATP concentrations as indicated. b pH titration: The graph represents the light intensity of each reaction at different pH. c Luciferin titration: The graph represents the light intensity of each reaction using different luciferase concentrations as indicated. Graphs A, B and C are in logarithm scale. d Light emission decay time: The graph represents the time that the red luciferase emits light. The means and standard deviations of all graphs were obtained from quadruplicate experiments

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BMC Biochemistry

ISSN: 1471-2091

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