Fig. 2From: Dacin, one metalloproteinase from Deinagkistrodon acutus venom inhibiting contraction of mouse ileum muscleSDS-PAGE and HPLC analysis of Dacin. a, 12% SDS-PAGE spectrums of Dacin. Lane 1, Dacin under reducing conditions; MW lane, standard protein markers (kD); lane 2, Dacin under non-reducing conditions. b, Chromatogram of Dacin sample on a analytical C18 column of HPLC, equilibrated with buffer A (0.1% TFA) and eluted with the following gradient of buffer B (80% acetonitrile in 0.1% TFA) and buffer A at a rate of 1 ml/minBack to article page