Fig. 1

Chromatograms of Dacin isolated and purified from D. acutus venom and its SDS-PAGE identification. a, Chromatogram of 200 mg D. acutus venom on Sephadex G-50 column (1.1 cm × 100 cm), eluted with 50 mM Tris-HCl buffer (pH 8.4) at a rate of 0.15 ml/min. Peak II showed Dacin’s activity. b, Chromatogram of Peak II on DEAE Sepharose Fast Flow ion-exchange column (1.6 × 20 cm), eluted with 50 mM Tris-HCl buffer (pH 8.4) at a rate of 1.5 ml/min, then combined with a linear gradient NaCl (0-0.2 M) elution. Peak 6 presented Dacin’s activity. c, Chromatogram of Peak 6 on Hitrap Capto DEAE ion-exchange column (0.7 × 2.5 cm). Peak b exhibited Dacin’s activity. d 15% SDS-PAGE analysis of D. acutus venom and Dacin. MW lane, standard protein markers (kD); lane 1, Peak b; lane 2, Peak 6; lane 3, Peak II; lane 4, D. acutus venom