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Table 1 Comparison of Tm values for ScTrl1 PNK/CPDase, ScTrl1 CPDase, and BfPNK/CPDase. The highest Tm values are indicated in bold

From: Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase

Protein

Buffer condition

Tm (°C)

ScTrl1 PNK/CPDase

50 mM HEPES, pH 7.5, 150 mM NaCl

55

50 mM imidazole, pH 8.0, 150 mM NaCl

50

50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM 2′,3′-cNADP+

63

ScTrl1 CPDase

50 mM HEPES, pH 7.5, 150 mM NaCl

58

50 mM imidazole, pH 8.0, 150 mM NaCl

55

50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM 2′,3′-cNADP+

72

BfPNK/CPDase

50 mM HEPES, pH 7.5, 150 mM NaCl

43

50 mM MES, pH 7.0, 500 150 mM NaCl

43

50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM 2′,3′-cNADP+

48

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BMC Biochemistry

ISSN: 1471-2091

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