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Table 1 Comparison of Tm values for ScTrl1 PNK/CPDase, ScTrl1 CPDase, and BfPNK/CPDase. The highest Tm values are indicated in bold

From: Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase

Protein Buffer condition Tm (°C)
ScTrl1 PNK/CPDase 50 mM HEPES, pH 7.5, 150 mM NaCl 55
50 mM imidazole, pH 8.0, 150 mM NaCl 50
50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM 2′,3′-cNADP+ 63
ScTrl1 CPDase 50 mM HEPES, pH 7.5, 150 mM NaCl 58
50 mM imidazole, pH 8.0, 150 mM NaCl 55
50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM 2′,3′-cNADP+ 72
BfPNK/CPDase 50 mM HEPES, pH 7.5, 150 mM NaCl 43
50 mM MES, pH 7.0, 500 150 mM NaCl 43
50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM 2′,3′-cNADP+ 48
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BMC Biochemistry

ISSN: 1471-2091

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