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Fig. 4 | BMC Biochemistry

Fig. 4

From: Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase

Fig. 4

PNK/CPDase interacts with E. coli RNA. a Superdex 200 SEC profile of ScTrl1 PNK/CPDase (continuous lines) and BfPNK/CPDase (dashed lines). The peaks close to the void volume with A260 (green) higher than A280 (blue) indicate the presence of a nucleic acid. VO: column void volume. b Agarose gels of BfPNK/CPDase and ScTrl1 PNK/CPDase. L: DNA ladder, 1: The protein-nucleic acid complex eluted near the void volume from SEC, 2: DNase-treated protein-nucleic acid complex, 3: RNase-treated protein-nucleic acid complex. RNase degrades the nucleic acid, confirming that the co-purified molecule is, in fact, RNA

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BMC Biochemistry

ISSN: 1471-2091

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